Guangxi TCM Resources General Survey and Data Collection Key Laboratory, Guangxi Botanical Garden of Medicinal Plants, Nanning, 530023, China.
Guangxi Medicinal Resources Conservation and Genetic Improvement Key Laboratory, Guangxi Botanical Garden of Medicinal Plants, 530023, Nanning, China.
J Plant Physiol. 2023 Jul;286:154007. doi: 10.1016/j.jplph.2023.154007. Epub 2023 May 12.
Seed dormancy and germination are critical to medicinal plant reproduction. Dormancy-associated gene (DRM1) has been involved in the regulation of dormancy in Arabidopsis meristematic tissues or organs. However, research on molecular functions and regulations of DRM1 in Amomum tsaoko, an important medicinal plant, is rare. In this study, the DRM1 was isolated from embryos of A. tsaoko, and the results of protein subcellular localization in Arabidopsis protoplast indicated that DRM1 was mainly nucleus and cytoplasm. Expression analysis showed that DRM1 especially exhibited the highest transcript level in dormant seed and short-time stratification while displaying a high response of hormone and abiotic stress. Further investigation showed that ectopic expression of DRM1 in Arabidopsis exhibited delayed seed germination and germination capability to high temperatures. Additionally, DRM1 transgenic Arabidopsis exhibited increased tolerance to heat stress by enhancing antioxidative capacities and regulating stress-associated genes (AtHsp25.3-P, AtHsp18.2-CI, AtHsp70B, AtHsp101, AtGolS1, AtMBF1c, AtHsfA2, AtHsfB1 and AtHsfB2). Overall, our results reveal the role of DRM1 in seed germination and abiotic stress response.
种子休眠和萌发对药用植物的繁殖至关重要。休眠相关基因 (DRM1) 已参与拟南芥分生组织组织或器官休眠的调节。然而，关于药用植物砂仁中 DRM1 的分子功能和调控的研究很少。本研究从砂仁胚中分离出 DRM1，亚细胞定位结果表明 DRM1 主要位于细胞核和细胞质中。表达分析表明，DRM1 特别是在休眠种子和短时间层积中表现出最高的转录水平，对激素和非生物胁迫有较高的响应。进一步的研究表明，DRM1 在拟南芥中的异位表达导致种子萌发延迟和对高温的萌发能力降低。此外，DRM1 转基因拟南芥通过增强抗氧化能力和调节应激相关基因（AtHsp25.3-P、AtHsp18.2-CI、AtHsp70B、AtHsp101、AtGolS1、AtMBF1c、AtHsfA2、AtHsfB1 和 AtHsfB2）提高了对热应激的耐受性。总体而言，我们的研究结果揭示了 DRM1 在种子萌发和非生物胁迫响应中的作用。
