College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 225108, China; Jiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 225108, China.
Poult Sci. 2023 Jul;102(7):102736. doi: 10.1016/j.psj.2023.102736. Epub 2023 Apr 20.
Quercetin (Que), widely found in a huge variety of plants, plays important roles in ovarian function. However, to data, there have been no reports about Que regulating granulosa cells (GCs) in prehierarchical follicles in chicken. Herein, GCs from follicles diameter from 4 to 8 mm in chicken were treated by Que in vitro culture to investigate how Que exerts its effect on follicular development. GCs treated by Que in concentrations of 10, 100, and 1,000 ng/mL were tested for cell proliferation and progesterone secretion. Eight cDNA libraries were constructed from GCs (4 samples per group) to explore transcriptome expression changes. The role of the MAPK/ERK signaling pathway was validated in this process. Treatment with 100 and 1,000 ng/mL levels of Que significantly promoted cell proliferation and progesterone secretion (P < 0.05). RNA-seq analysis data showed that 402 and 263 differentially expressed genes (DEGs) were up- and down-regulated, respectively. Functional enrichment analysis that the pathways related to follicular development included biosynthesis of amino acids, MAPK signaling pathway, and calcium signaling pathway. Notably, the function exerted in GCs of the different levels of Que was associated with the suppression of the MAPK pathway. In conclusion, our results proved that low levels of Que could promote MAPK signaling pathway, but high levels of Que inhibit MAPK signaling pathway in GCs from the prehierarchical follicles, promote cell proliferation and progesterone secretion, and benefit follicle selection.
槲皮素（Que）广泛存在于多种植物中，在卵巢功能中发挥着重要作用。然而，迄今为止，尚无关于 Que 调节鸡前卵泡颗粒细胞（GCs）的报道。在此，我们通过体外培养的方式用 Que 处理直径为 4 至 8mm 的鸡卵泡中的 GCs，以研究 Que 如何对卵泡发育发挥作用。我们用浓度为 10、100 和 1000ng/ml 的 Que 处理 GCs，检测细胞增殖和孕激素分泌情况。我们从 GCs（每组 4 个样本）构建了 8 个 cDNA 文库，以探索转录组表达变化。在此过程中，我们验证了 MAPK/ERK 信号通路的作用。用 100 和 1000ng/ml 的 Que 处理可显著促进细胞增殖和孕激素分泌（P＜0.05）。RNA-seq 分析数据显示，分别有 402 个和 263 个差异表达基因（DEGs）上调和下调。功能富集分析表明，与卵泡发育相关的通路包括氨基酸生物合成、MAPK 信号通路和钙信号通路。值得注意的是，Que 在不同水平下对 GCs 的功能与 MAPK 通路的抑制有关。综上所述，我们的研究结果证明，低水平的 Que 可以促进 MAPK 信号通路，但高水平的 Que 抑制前卵泡 GCs 中的 MAPK 信号通路，促进细胞增殖和孕激素分泌，有利于卵泡选择。
