Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Key Laboratory of Chemical Biology and Molecular Engineering, Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Oct 15;299:122823. doi: 10.1016/j.saa.2023.122823. Epub 2023 May 9.
Hypochlorite (ClO) is an important bioactive molecule of living system which plays essential roles in many physiological and pathological processes. There is no doubt that the biological roles of ClO depend highly on the concentration of ClO. Unfortunately, the relationship between the concentration of ClO and the biological process is unclear. Toward this purpose, in this work we addressed a core challenge for developing a powerful fluorescence tool for monitoring a wide concentration change (0-14 eq.) of ClO via two distinct detection manners. The probe displayed fluorescence variation (red to green) upon addition of ClO (0-4 eq.) and the color of test medium changed from red to colorless witnessed by the naked eyes. Surprisingly, in the presence of higher concentration of ClO (4-14 eq.), the probe displayed another fluorescent signal change from green to blue. After demonstrating the excellent sensing properties of the probe with ClO in vitro, it was successfully used to imaging different concentration of ClO in living cells. We expected that the probe could act as an exciting chemistry tool for imaging of ClO concentration-dependent oxidative stress event in biological system.
次氯酸根（ClO）是生命体系中的一种重要活性分子，在许多生理和病理过程中发挥着重要作用。毫无疑问，ClO 的生物学作用高度依赖于 ClO 的浓度。不幸的是，ClO 浓度与生物学过程之间的关系尚不清楚。针对这一目的，在这项工作中，我们通过两种不同的检测方式解决了开发用于监测 ClO 广泛浓度变化（0-14eq.）的强大荧光工具的核心挑战。探针在加入 ClO（0-4eq.）时显示荧光变化（红色变为绿色），并且测试介质的颜色肉眼可见地从红色变为无色。令人惊讶的是，在存在较高浓度的 ClO（4-14eq.）时，探针显示出另一种从绿色变为蓝色的荧光信号变化。在体外用 ClO 证明了探针的优异传感性能后，它成功地用于活细胞中不同浓度的 ClO 的成像。我们期望该探针可以作为一种令人兴奋的化学工具，用于在生物体系中成像 ClO 浓度依赖性氧化应激事件。
