Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile.
Microbiota-Host Interactions and Clostridia Research Group, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile; ANID-Millennium Science Initiative Program-Millennium Nucleus in the Biology of the Intestinal Microbiota, Santiago, Chile.
J Glob Antimicrob Resist. 2023 Jun;33:328-336. doi: 10.1016/j.jgar.2023.05.004. Epub 2023 May 19.
Clostridioides difficile is a nosocomial pathogen that is associated with the use of antibiotics. One of the most worrying aspects of C. difficile infection is its ability to resist antimicrobial therapies, owing to spore formation. In several bacterial pathogens, proteases of the Clp family participate in phenotypes associated with persistence and virulence. This suggests that these proteins could be involved in virulence-related traits. In this study, we analysed the role of ClpC chaperone-protease of C. difficile in virulence-related traits by comparing the phenotypes of wild-type and mutant strains lacking the clpC gene (ΔclpC).
We performed biofilm, motility, spore formation, and cytotoxicity assays.
Our results show significant differences between the wild-type and ΔclpC strains in all analysed parameters.
Based on these findings, we conclude that clpC plays a role in the virulence properties of C. difficile.
艰难梭菌是一种与抗生素使用相关的医院病原体。艰难梭菌感染最令人担忧的一个方面是其能够通过形成孢子来抵抗抗菌治疗。在几种细菌病原体中，Clp 家族的蛋白酶参与与持续存在和毒力相关的表型。这表明这些蛋白质可能与毒力相关的性状有关。在这项研究中，我们通过比较缺乏 clpC 基因（ΔclpC）的野生型和突变菌株的表型，分析了艰难梭菌 ClpC 伴侣蛋白酶在与毒力相关的性状中的作用。
我们进行了生物膜、运动性、孢子形成和细胞毒性测定。
我们的结果表明，在所有分析的参数中，野生型和ΔclpC 菌株之间存在显著差异。
基于这些发现，我们得出结论，clpC 基因在艰难梭菌的毒力特性中发挥作用。
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