乳腺癌导管癌中 及 miR - 132 的下调。 （注：原文中“Down - regulation of and miR - 132”里第一个“of”后面缺少具体内容）
Down-regulation of and miR-132 in ductal carcinoma of the breast.
作者信息
Abbasi-Kolli Mohammad, Shahbazi Shirin, Geranpayeh Loabat
机构信息
Department of Medical Genetics, Faculty of Medical Sciences, Tarbiat Modares University Tehran, Iran.
Department of Surgery, Sina Hospital, Tehran University of Medical Sciences Tehran, Iran.
出版信息
Int J Mol Epidemiol Genet. 2023 Apr 15;14(1):1-10. eCollection 2023.
INTRODUCTION
miR-132-3p acts in normal breast development and its downregulation has been documented in breast cancer. One of the targets of miR-132-3p is which is also inactivated in breast cancer. The interactions between and miR-132 have been reported in several pathological conditions. We aimed to investigate the correlation between expression levels of miR-132 and in ductal carcinoma of the breast.
METHODS
The study was carried out on tissues obtained from female patients with primary breast cancer. Tumor samples were classified using clinical and pathological data. Following RNA extraction and cDNA synthesis, relative gene expressions in tumors were compared to non-cancerous adjacent tissues. The link between and miR-132 was assessed by the correlation coefficient test.
RESULTS
Our findings revealed a significant decrease in miR-132 and expressions with a ratio of 0.165 and 0.365, respectively. Tumor grade showed an association with miRNA-132 levels. The expression of miR-132 in grade I tumors was almost equal to that of normal adjacent tissues, but was intensely decreased in grades II and III. The correlation analysis showed a small linear association between and miR-132 levels.
CONCLUSION
The reduction of miR-132 and expression confirmed the tumor-suppressive role of both genes in breast cancer. Considering that is one of the miR-132 targets, further studies are required to discover any miRNA-mediated upregulation role for miR-132. Our finding discovered a small linear association between miR-132 and , which can be concluded towards their independent function in breast cancer pathogenesis.
引言
miR-132-3p在正常乳腺发育中发挥作用，且在乳腺癌中其表达下调已得到证实。miR-132-3p的靶标之一在乳腺癌中也处于失活状态。在几种病理状况下，已报道了与miR-132之间的相互作用。我们旨在研究乳腺导管癌中miR-132和的表达水平之间的相关性。
方法
本研究对原发性乳腺癌女性患者的组织进行。利用临床和病理数据对肿瘤样本进行分类。在提取RNA并合成cDNA后，将肿瘤中的相对基因表达与癌旁非癌组织进行比较。通过相关系数检验评估与miR-132之间的联系。
结果
我们的研究结果显示，miR-132和表达显著降低，其比值分别为0.165和0.365。肿瘤分级与miRNA-132水平相关。I级肿瘤中miR-132的表达几乎与癌旁正常组织相等，但在II级和III级中显著降低。相关性分析显示与miR-132水平之间存在较小的线性关联。
结论
miR-132和表达的降低证实了这两个基因在乳腺癌中的肿瘤抑制作用。鉴于是的miR-132靶标之一，需要进一步研究以发现miR-132的任何miRNA介导的上调作用。我们的研究发现miR-132和之间存在较小的线性关联，由此可推断它们在乳腺癌发病机制中具有独立功能。
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