用于噬菌体敏感性研究的动物源产超广谱β-内酰胺酶和AmpCβ-内酰胺酶共生菌代表性菌株集。
A Representative Collection of Commensal Extended-Spectrum- and AmpC-β-Lactamase-Producing of Animal Origin for Phage Sensitivity Studies.
作者信息
Vitt Amira R, Sørensen Martine C Holst, Bortolaia Valeria, Brøndsted Lone
机构信息
Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg C, Denmark.
Department of Bacteria, Parasites and Fungi, Statens Serum Institut, Copenhagen S, Denmark.
出版信息
Phage (New Rochelle). 2023 Mar 1;4(1):35-45. doi: 10.1089/phage.2023.0002. Epub 2023 Mar 17.
INTRODUCTION
Extended-spectrum β-lactamase (ESBL)- and AmpC β-lactamase (AmpC)-producing from livestock and meat represent a zoonotic risk and biocontrol solutions are needed to prevent transmission to humans.
METHODS
In this study, we established a representative collection of animal-origin ESBL/AmpC as target to test the antimicrobial potential of bacteriophages.
RESULTS
Bioinformatic analysis of whole-genome sequence data of 198 ESBL/AmpC from pigs, broilers, and broiler meat identified strains belonging to all known phylogroups and 65 multilocus sequence types. Various ESBL/AmpC genes and plasmid types were detected with expected source-specific patterns. Plaque assay using 15 phages previously isolated using the reference collection demonstrated that phages showed the broadest host range, killing up to 26 strains.
CONCLUSIONS
154/198 strains were resistant to infection by all phages tested, suggesting a need for isolating phages specific for ESBL/AmpC . The strain collection described in this study is a useful resource fulfilling such need.
引言
家畜和肉类中产生超广谱β-内酰胺酶（ESBL）和AmpCβ-内酰胺酶（AmpC）的细菌存在人畜共患病风险，需要生物防治解决方案来防止其传播给人类。
方法
在本研究中，我们建立了一个具有代表性的动物源ESBL/AmpC细菌集合作为测试噬菌体抗菌潜力的目标。
结果
对来自猪、肉鸡和鸡肉的198株ESBL/AmpC细菌的全基因组序列数据进行生物信息学分析，确定了属于所有已知系统发育群和65种多位点序列类型的菌株。检测到各种ESBL/AmpC基因和质粒类型，并呈现出预期的来源特异性模式。使用先前从参考集合中分离出的15种噬菌体进行噬菌斑测定，结果表明一种噬菌体显示出最广泛的宿主范围，可杀死多达26株菌株。
结论
198株菌株中有154株对所有测试噬菌体的感染具有抗性，这表明需要分离针对ESBL/AmpC细菌的特异性噬菌体。本研究中描述的菌株集合是满足此类需求的有用资源。
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