State Key Laboratory of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; Joint International Research Laboratory of Food Safety, Jiangnan University, No.1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; Engineering Research Center of Dairy Quality and Safety Control Technology, Ministry of Education, Inner Mongolia University, No.235 Daxue West Road, Hohhot 010021, Inner Mongolia Autonomous Region, China; School of Food Science and Technology, Jiangnan University, No.1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China; Joint International Research Laboratory of Food Safety, Jiangnan University, No.1800 Lihu Avenue, Wuxi 214122, Jiangsu Province, China.
Int J Biol Macromol. 2023 Jul 1;242(Pt 3):124937. doi: 10.1016/j.ijbiomac.2023.124937. Epub 2023 May 20.
Nanoplastics could modulate the fibrillation of amyloid proteins. However, many chemical functional groups are adsorbed to change the interfacial chemistry of nanoplastics in the real world. Herein, this study aimed to investigate the effects of polystyrene (PS), carboxyl modified PS (PS-COOH), and amino modified PS (PS-NH) on the fibrillation of hen egg-white lysozyme (HEWL). Due to the differences in the interfacial chemistry, concentration was considered an essential factor. PS-NH (10 μg/mL) could promote the fibrillation of HEWL similar to PS (50 μg/mL) and PS-COOH (50 μg/mL). Moreover, promoting the primary nucleation step of amyloid fibril formation was the primary reason. The differences in spatial conformation of HEWL were characterized by Fourier transform-infrared spectroscopy and surface enhanced Raman spectroscopy (SERS). Strikingly, a particular signal of SERS of HEWL incubated with PS-NH at 1610 cm was found due to the interaction between amino group of PS-NH and tryptophan (or tyrosine) of HEWL. Therefore, a new perspective was provided to understand the regulation of interfacial chemistry of nanoplastics on the fibrillation of amyloid proteins. Additionally, this study suggested that SERS could be a powerful method to investigate the interactions between proteins and nanoparticles.
纳米塑料可以调节淀粉样蛋白的纤维形成。然而，在现实世界中，许多化学官能团被吸附，从而改变了纳米塑料的界面化学性质。本研究旨在探讨聚苯乙烯（PS）、羧基化 PS（PS-COOH）和氨基化 PS（PS-NH）对鸡蛋清溶菌酶（HEWL）纤维形成的影响。由于界面化学性质的差异，浓度被认为是一个重要因素。PS-NH（10μg/mL）能够像 PS（50μg/mL）和 PS-COOH（50μg/mL）一样促进 HEWL 的纤维形成。此外，促进淀粉样纤维形成的初级成核步骤是主要原因。傅里叶变换红外光谱和表面增强拉曼光谱（SERS）表征了 HEWL 的空间构象差异。值得注意的是，在 PS-NH 存在下孵育的 HEWL 的 SERS 中发现了一个特殊的 1610cm 处信号，这归因于 PS-NH 的氨基与 HEWL 中的色氨酸（或酪氨酸）之间的相互作用。因此，为理解纳米塑料的界面化学对淀粉样蛋白纤维形成的调节提供了新的视角。此外，本研究表明 SERS 可以成为研究蛋白质与纳米颗粒相互作用的有力方法。
