Institute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
Institute of Medicinal Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
Genomics. 2023 Jul;115(4):110643. doi: 10.1016/j.ygeno.2023.110643. Epub 2023 May 20.
MYB family is one of the largest transcription factor families in plants and plays a crucial role in regulating plant biochemical and physiological processes. However, R2R3-MYBs in patchouli have not been systematically investigated. Here, based on the gene annotation of patchouli genome sequence, 484 R2R3-MYB transcripts were detected. Further in-depth analysis of the gene structure and expression of R2R3-MYBs supported the tetraploid hybrid origin of patchouli. When combined with R2R3-MYBs from Arabidopsis, a phylogenetic tree of patchouli R2R3-MYBs was constructed and divided into 31 clades. Interestingly, a patchouli-specific R2R3-MYB clade was found and confirmed by homologous from other Lamiaceae species. The syntenic analysis demonstrated that tandem duplication contributed to its evolution. This study systematically analysed the R2R3-MYB family in patchouli, providing information on its gene characterization, functional prediction, and species evolution.
MYB 家族是植物中最大的转录因子家族之一，在调节植物生化和生理过程中起着至关重要的作用。然而，在广藿香中，R2R3-MYB 尚未得到系统研究。在这里，基于广藿香基因组序列的基因注释，检测到 484 个 R2R3-MYB 转录本。对 R2R3-MYBs 的基因结构和表达的进一步深入分析支持了广藿香的四倍体杂种起源。当与拟南芥的 R2R3-MYBs 结合时，构建了广藿香 R2R3-MYBs 的系统发育树，并分为 31 个分支。有趣的是，发现了一个广藿香特有的 R2R3-MYB 分支，并通过其他唇形科物种的同源性得到了证实。共线性分析表明串联重复有助于其进化。本研究系统地分析了广藿香中的 R2R3-MYB 家族，提供了其基因特征、功能预测和物种进化的信息。
