School of Food and Advanced Technology, College of Sciences, Massey University, Riddet Complex A Building, Riddet Road, Palmerston North 4410, New Zealand.
Cinder Biological, Inc. (CinderBio), 1933 Davis St. Suite 208, San Leandro, CA 94577, USA.
J Appl Microbiol. 2023 Jun 1;134(6). doi: 10.1093/jambio/lxad106.
To test the efficacy of novel hot/acid hyperthermoacidic enzyme treatments on the removal of thermophilic spore-forming biofilms from stainless steel surfaces.
The present study measured the efficacy of hyperthermoacidic enzymes (protease, amylase, and endoglucanase) that are optimally active at low pH (≈3.0) and high temperatures (≈80°C) at removing thermophilic bacilli biofilms from stainless steel (SS) surfaces. Plate counts, spore counts, impedance microbiology, as well as epifluorescence microscopy, and scanning electron microscopy (SEM) were used to evaluate the cleaning and sanitation of biofilms grown in a continuous flow biofilm reactor. Previously unavailable hyperthermoacidic amylase, protease, and the combination of amylase and protease were tested on Anoxybacillus flavithermus and Bacillus licheniformis, and endoglucanase was tested on Geobacillus stearothermophilus. In all cases, the heated acidic enzymatic treatments significantly reduced biofilm cells and their sheltering extracellular polymeric substances (EPS).
Hyperthermoacidic enzymes and the associated heated acid conditions are effective at removing biofilms of thermophilic bacteria from SS surfaces that contaminate dairy plants.
测试新型热/酸嗜热酶处理对不锈钢表面耐热孢子形成生物膜的去除效果。
本研究测量了在低 pH（≈3.0）和高温（≈80°C）下最活跃的嗜热酶（蛋白酶、淀粉酶和内切葡聚糖酶）从不锈钢（SS）表面去除耐热杆菌生物膜的效果。平板计数、孢子计数、阻抗微生物学以及荧光显微镜和扫描电子显微镜（SEM）用于评估在连续流动生物膜反应器中生长的生物膜的清洁和卫生情况。以前不可用的嗜热淀粉酶、蛋白酶以及淀粉酶和蛋白酶的组合被测试用于黄杆菌属（Anoxybacillus flavithermus）和地衣芽孢杆菌（Bacillus licheniformis），内切葡聚糖酶被测试用于嗜热脂肪地芽孢杆菌（Geobacillus stearothermophilus）。在所有情况下，加热酸性酶处理显著减少了生物膜细胞及其遮蔽的胞外聚合物物质（EPS）。
嗜热酶和相关的加热酸条件可有效去除污染乳制品厂的 SS 表面的耐热细菌生物膜。
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