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乳制品工业中的生物膜:表面材料、培养温度和牛奶类型的影响。

and biofilms in the dairy industry: effects of surface material, incubation temperature and milk type.

机构信息

Department of Biology, Faculty of Science, Ankara University , Ankara Turkey.

Department of Food Engineering, Faculty of Engineering and Architecture, Konya Food and Agriculture University , Konya , Turkey.

出版信息

Biofouling. 2019 May;35(5):551-560. doi: 10.1080/08927014.2019.1628221. Epub 2019 Jul 5.

Abstract

(. , . subsp. asachharedens, . and . ) and (two strains of . , . and . ) isolates and reference strains in whole milk were evaluated for their biofilm production on six different abiotic surfaces. DSM 465 had the highest cell counts (>4 log CFU cm) on glass and stainless steel (SS) at 55 and 65 °C, respectively. D195 had the highest counts on SS at 55 °C (>5 log CFU cm) and polyvinyl chloride (PVC) at 65 °C (>4 log CFU cm), indicating the existence of strain variation. The ideal surfaces for all strains were SS and glass at 55 °C, but their preferences were polystyrene and SS at 65 °C. Moreover, members were more prone to form biofilms in skim milk than in semi-skim and whole milk, whereas the results were the opposite for . Both the attachment and sporulation of in whole milk was higher than in semi-skim or skim milk. This study proposes that the surface material, temperature and milk type had a cumulative effect on biofilm formation.

摘要

(. ,. 亚种. ,. 和. )以及 (两种. ,. 和. 菌株)在全脂牛奶中的生物膜形成能力在六种不同的非生物表面上进行了评估。在 55 和 65°C 下,DSM 465 在玻璃和不锈钢(SS)上的细胞计数最高(>4 log CFU cm)。D195 在 55°C 时 SS 上的计数最高(>5 log CFU cm)和聚氯乙烯(PVC)上的计数最高(>4 log CFU cm),表明存在菌株变异。所有菌株的理想表面均为 55°C 时的 SS 和玻璃,但它们在 65°C 时更喜欢聚苯乙烯和 SS。此外,. 成员在脱脂奶中比在半脱脂和全脂奶中更容易形成生物膜,而. 的结果则相反。. 在全脂牛奶中的附着和孢子形成均高于半脱脂或脱脂牛奶。本研究表明,表面材料、温度和牛奶类型对生物膜形成有累积效应。

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