College of Chemical and Biological Engineering, Shandong University of Science and Technology, Qingdao, Shandong, P. R. China.
College of Life Sciences, State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest Agriculture & Forestry University, Yangling, Shannxi, P. R. China.
Mol Biol Rep. 2023 Jul;50(7):5777-5789. doi: 10.1007/s11033-023-08485-3. Epub 2023 May 23.
Promoters play key roles in plant gene expression in complex and varied natural environments. The type and amount of cis-acting elements in the promoter sequence tend to indicate the response of genes to induction factors. WRAB18 is a group III member of the late embryogenesis abundant (LEA) protein family that performs multiple functions in plant stress physiology. To elucidate the particularly biological effects of WRAB18 on stress, exploration of its promoter sequence is necessary.
In this study, the full-length and promoter sequences of Wrab18 were isolated from the Zhengyin 1 cultivar of Triticum aestivum. The gene sequences and cis-acting elements in the promoter were analyzed using the Plant Promoter Database and bioinformatics methods. The results showed that Wrab18 possessed one intron with 100 bp, the promoter sequence contained various stress-related cis-acting elements, and the functionality of the promoter was checked using green fluorescent protein (GFP) marker protein expression by transient assay in Nicotiana benthamiana. Furthermore, based on promoter prediction analysis, quantitative real-time fluorescent PCR results confirmed the response of gene expression levels to stress factors.
In summary, the promoter sequence of Wrab18 plays a role in plant stress responses, contains multiple cis-acting elements, and provides insights into the role of WRAB18 in plant resilience to stress. This study has guiding significance for further studies of gene function and mechanism of action, and lays a theoretical foundation for improving wheat quality.
启动子在复杂多样的自然环境中对植物基因表达起着关键作用。启动子序列中的顺式作用元件的类型和数量往往表明基因对诱导因子的反应。WRAB18 是晚期胚胎丰富（LEA）蛋白家族的 III 组成员，在植物应激生理中具有多种功能。为了阐明 WRAB18 对胁迫的特殊生物学效应，有必要对其启动子序列进行探索。
本研究从冬小麦品种珍银 1 中分离出 Wrab18 的全长和启动子序列。使用 Plant Promoter Database 和生物信息学方法分析基因序列和启动子中的顺式作用元件。结果表明，Wrab18 具有一个 100bp 的内含子，启动子序列包含各种与应激相关的顺式作用元件，并通过瞬时测定在烟草原生质体中使用绿色荧光蛋白（GFP）标记蛋白表达来检查启动子的功能。此外，基于启动子预测分析，定量实时荧光 PCR 结果证实了基因表达水平对胁迫因子的响应。
综上所述，Wrab18 的启动子序列在植物应激反应中起作用，包含多个顺式作用元件，为 WRAB18 在植物对胁迫的恢复力中的作用提供了深入了解。本研究对进一步研究基因功能和作用机制具有指导意义，并为提高小麦品质奠定了理论基础。
