Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona, Spain.
Department of Plant Biology, University of Illinois Urbana-Champaign, Champaign, IL, USA.
J Exp Bot. 2023 Jul 18;74(13):3821-3832. doi: 10.1093/jxb/erad172.
Protoplasts, which are plant cells with their cell walls removed, have been used for decades in plant research and have been instrumental in genetic transformation and the study of various aspects of plant physiology and genetics. With the advent of synthetic biology, these individualized plant cells are fundamental to accelerate the 'design-build-test-learn' cycle, which is relatively slow in plant research. Despite their potential, challenges remain in expanding the use of protoplasts in synthetic biology. The capacity of individual protoplasts to hybridize to form new varieties, and to regenerate from single cells, creating individuals with new features is underexplored. The main objective of this review is to discuss the use of protoplasts in plant synthetic biology and to highlight the challenges to exploiting protoplast technologies in this new 'age of synthetic biology'.
原生质体是去除细胞壁的植物细胞，已经在植物研究中使用了几十年，在遗传转化和研究植物生理学和遗传学的各个方面都发挥了重要作用。随着合成生物学的出现，这些个体化的植物细胞对于加速“设计-构建-测试-学习”周期至关重要，而这个周期在植物研究中相对较慢。尽管有潜力，但在扩大原生质体在合成生物学中的应用方面仍然存在挑战。单个原生质体杂交形成新品种的能力，以及从单个细胞再生，创造具有新特征的个体的能力，还没有得到充分探索。本文的主要目的是讨论原生质体在植物合成生物学中的应用，并强调在这个新的“合成生物学时代”利用原生质体技术所面临的挑战。
