脂肪细胞因子表达调节性 T 细胞通过细胞吞噬现象对三阴性乳腺癌细胞的潜在作用。
Potential activity of adiponectin-expressing regulatory T cells against triple-negative breast cancer cells through the cell-in-cell phenomenon.
机构信息
Department of Gastroenterological Surgery and Pediatric Surgery, Gifu University Graduate School of Medicine, Gifu, Japan.
Department of Pathology and Translational Research, Gifu University Graduate School of Medicine, Gifu, Japan.
出版信息
Thorac Cancer. 2023 Jul;14(20):1941-1945. doi: 10.1111/1759-7714.14940. Epub 2023 May 23.
BACKGROUND
A population of regulatory T cells (Treg), which reside within thymic nurse cell complexes, express adiponectin and abrogate breast cancer development in transgenic mice. In this study, we examined whether adiponectin-expressing Treg could impair triple-negative breast cancer, which is defined by a lack of estrogen receptors, progesterone receptors, and human epidermal growth factor receptor-2.
METHODS
CD4- and CD25-positive cells were sorted from cultured T lymphocytes of a previously characterized experimental thymic tumor model composed of thymic nurse cells and abundant lymphoid stroma. These sorted cells were examined for FOXP3 and adiponectin immunoreactivity and subsequently exposed to triple-negative breast cancer MDA-MB-157 and -231 cells.
RESULTS
Adiponectin-expressing Treg were obtained by CD4- and CD25-positive sorting and cell death was induced in triple-negative breast cancer cells through the cell-in-cell phenomenon.
CONCLUSIONS
Adiponectin-expressing Treg may be candidates for adoptive cell therapy against triple-negative breast cancer.
背景
存在于胸 腺 保姆细胞复合物中的调节性 T 细胞（Treg）群体表达脂联素，并在转基因小鼠中消除乳腺癌的发生。在本研究中，我们研究了表达脂联素的 Treg 是否可以破坏三阴性乳腺癌，三阴性乳腺癌的定义是缺乏雌激素受体、孕激素受体和人表皮生长因子受体 2。
方法
从先前建立的包含胸腺保姆细胞和丰富的淋巴基质的实验性胸腺肿瘤模型培养的 T 淋巴细胞中分离出 CD4 和 CD25 阳性细胞。对这些分离的细胞进行 FOXP3 和脂联素免疫反应性检测，然后将其暴露于三阴性乳腺癌 MDA-MB-157 和 MDA-MB-231 细胞中。
结果
通过 CD4 和 CD25 阳性分选获得了表达脂联素的 Treg，并且通过细胞内-细胞现象诱导三阴性乳腺癌细胞死亡。
结论
表达脂联素的 Treg 可能是针对三阴性乳腺癌的过继细胞治疗的候选者。
Zotero 插件