Department of Microbiology, University of Balochistan, Quetta, 87300, Balochistan, Pakistan.
Department of Botany, University of Balochistan, Quetta, Balochistan, Pakistan.
Sci Rep. 2023 May 23;13(1):8328. doi: 10.1038/s41598-023-34893-3.
Vitis vinifera L., commonly known as grape is a major fruit crop in the world. Grapes seem to confer health benefits due to their chemical components, biological and antioxidant activities. The present study is conducted to evaluate the biochemical constituents, antioxidant, and antimicrobial potential of ethanolic grape peduncles (EGP) extract. The result of phytochemical analysis revealed the presence of various phytochemicals such as flavonoid, tannin, carbohydrates, alkaloids, cardiac glycoside, phenol, steroid, terpenoids, quinones and anthraquinones. Furthermore, total phenolic content (TPC) and total flavonoid contents (TFC) were 7.35 ± 0.25 mg GAE/g (Gallic Acid Equivalent per gram) and 29.67 ± 0.13 mg QE/g (Quercetin Equivalent per gram) respectively. DPPH (2, 2-diphenyl-1-picrylhydrazyl) free radical scavenging assay revealed IC = 159.3 μg/mL. The antibacterial and antifungal study disclosed that the extract was highly potent against Salmonella typhi with maximum zone of inhibition of 27.2 ± 1.60 mm and Epidermophyton floccosum with 74 ± 1.81% inhibition. The extract was analyzed for its cytotoxicity and antileishmanial activity and showed no activity against HeLa cell line and promastigotes of Leishmania major. Elements Fe, Mn, Ni, Pb and Cd were determined by atomic absorption spectroscopy and approximately 50 compounds were identified by Gas Chromatography-Mass Spectrometry (GC-MS). Current work suggest that grape peduncles can be a promising source of bioactive medicinal component.
葡萄，学名为 Vitis vinifera L.，是世界上主要的水果作物之一。由于其化学成分、生物和抗氧化活性，葡萄似乎具有健康益处。本研究旨在评估葡萄梗（EGP）乙醇提取物的生化成分、抗氧化和抗菌潜力。植物化学成分分析的结果表明，存在各种植物化学成分，如类黄酮、单宁、碳水化合物、生物碱、强心苷、酚类、类固醇、萜类、醌类和蒽醌类。此外，总酚含量（TPC）和总类黄酮含量（TFC）分别为 7.35±0.25 mg GAE/g（没食子酸当量/克）和 29.67±0.13 mg QE/g（槲皮素当量/克）。DPPH（2,2-二苯基-1-苦基肼基）自由基清除试验表明 IC=159.3μg/mL。抗菌和抗真菌研究表明，该提取物对伤寒沙门氏菌具有高度的抑菌活性，抑菌圈最大为 27.2±1.60mm，对絮状表皮癣菌的抑制率为 74±1.81%。对提取物进行细胞毒性和抗利什曼原虫活性分析，结果表明对 HeLa 细胞系和利什曼原虫前鞭毛体无活性。通过原子吸收光谱法测定了 Fe、Mn、Ni、Pb 和 Cd 等元素，通过气相色谱-质谱联用（GC-MS）鉴定了约 50 种化合物。目前的工作表明，葡萄梗可以成为有前途的生物活性药用成分来源。
