低温单分子荧光检测捕光复合物中固有色素的中红外响应。
Cryogenic Single-Molecule Fluorescence Detection of the Mid-Infrared Response of an Intrinsic Pigment in a Light-Harvesting Complex.
机构信息
Department of Physics, Tokyo Institute of Technology, Meguro, Tokyo 152-8550, Japan.
Department of Biochemistry and System Biomedicine, Graduate School of Medicine, Juntendo University, Bunkyo, Tokyo 113-8421, Japan.
出版信息
J Phys Chem B. 2023 Jun 8;127(22):4959-4965. doi: 10.1021/acs.jpcb.3c00284. Epub 2023 May 24.
We observed the mid-infrared (MIR) response of a single pigment of bacteriochlorophyll at the B800 binding site of a light-harvesting 2 complex. At a temperature of 1.5 K, a single complex in a spatially isolated spot in a near-infrared (NIR) fluorescence image was selected and was simultaneously irradiated with MIR and NIR light. We found that the temporal behavior of the NIR fluorescence excitation spectrum of individual pigments in a single complex was modulated by the MIR irradiation at 1650 cm. The MIR modulation of a single pigment was linearly proportional to the MIR intensity. The MIR linear response was detected in the range from 1580 to 1670 cm.
我们观察到了在一个光捕获 2 复合物的 B800 结合位点上的单个细菌叶绿素分子的中红外（MIR）响应。在 1.5 K 的温度下，在近红外（NIR）荧光图像中选择一个空间隔离点上的单个复合物，并同时用 MIR 和 NIR 光照射。我们发现，单个复合物中单个色素的 NIR 荧光激发光谱的时间行为被 1650 cm 的 MIR 辐射调制。单个色素的 MIR 调制与 MIR 强度呈线性比例关系。在 1580 至 1670 cm 的范围内检测到 MIR 线性响应。
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