270K微管相关蛋白与小龙虾外周神经轴突中抗MAP2 IgG发生交叉反应。
270K microtubule-associated protein cross-reacting with anti-MAP2 IgG in the crayfish peripheral nerve axon.
作者信息
Hirokawa N
出版信息
J Cell Biol. 1986 Jul;103(1):33-9. doi: 10.1083/jcb.103.1.33.
Abstract
MAPs (microtubule-associated proteins) were isolated from crayfish walking leg nerves. A major MAP was identified as a high molecular weight protein (270K). This protein co-migrated with mammalian MAP2, stimulated the polymerization of rat brain tubulin into microtubules, and was heat resistant. Rotary shadowing revealed that the 270K MAP is a long thin flexible structure. It formed cross-bridges of fine strands, linking microtubules with each other in vitro. These strands resemble the cross-bridges between microtubules observed in the crayfish axon permeabilized with saponin and quick-frozen, deep-etched. Antibodies against mammalian MAP2 cross-reacted with this crayfish MAP and stained the axoplasm of the walking leg nerves. Thus MAPs, especially the 270K MAP, appear to be a major component of the cross-linking strands between microtubules observed in the crayfish axon.
摘要
微管相关蛋白（MAPs）从小龙虾步足神经中分离得到。一种主要的MAP被鉴定为一种高分子量蛋白质（270K）。这种蛋白质与哺乳动物的微管相关蛋白2（MAP2）共同迁移，刺激大鼠脑微管蛋白聚合成微管，并且具有耐热性。旋转阴影法显示，270K MAP是一种细长的柔性结构。它形成了细丝状的交叉桥，在体外将微管相互连接。这些细丝类似于在用皂素通透并快速冷冻、深度蚀刻的小龙虾轴突中观察到的微管之间的交叉桥。抗哺乳动物MAP2的抗体与这种小龙虾MAP发生交叉反应，并对步足神经的轴质进行染色。因此，MAPs，尤其是270K MAP，似乎是在小龙虾轴突中观察到的微管之间交联细丝的主要成分。
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