Matus A, Bernhardt R, Hugh-Jones T
Proc Natl Acad Sci U S A. 1981 May;78(5):3010-4. doi: 10.1073/pnas.78.5.3010.
The distributions of tubulin and high molecular weight microtubule-associated proteins (HMWPs) in brain were determined by immunoperoxidase histochemistry with specific antisera. Tubulin was found in microtubules of both neurons and glia and both axons and dendrites. HMWPs were found only in neurons where, in all cases examined, they were associated with dendritic microtubules but not those in axons. Both tubulin and HMWPs were also found in postsynaptic densities. These results indicate that brain microtubules vary in chemical composition. The preferential association of HMWPs with dendritic microtubules suggests that they may play a role in distinguishing between dendritic and axonal export routes from the cell body.
利用特异性抗血清，通过免疫过氧化物酶组织化学法确定了微管蛋白和高分子量微管相关蛋白（HMWPs）在大脑中的分布。在神经元和神经胶质细胞的微管以及轴突和树突中均发现了微管蛋白。仅在神经元中发现了HMWPs，在所检查的所有情况下，它们都与树突状微管相关，而与轴突中的微管无关。在突触后致密物中也发现了微管蛋白和HMWPs。这些结果表明，脑微管的化学成分存在差异。HMWPs与树突状微管的优先结合表明，它们可能在区分从细胞体到树突和轴突的输出途径中发挥作用。
