Solid-supported reagents for the simultaneous extraction and derivatization of carboxylic acids from aqueous matrices. Studies on optimization of reaction conditions.

Solid-supported reagents for affecting a simultaneous extraction and derivatization of lipophilic and hydrophilic carboxylic acids from aqueous matrices were further investigated. The reagent consisted of pentafluorobenzyl bromide impregnated on the macroreticular resin XAD-2. New impregnation methods were developed which reduced the amount of pentafluorobenzyl bromide required and which were compatible with existing methods that utilize XAD-2 as an adsorbant. Factors affecting pentafluorobenzylation of these analytes were studied and the reaction conditions were optimized. Compatibility with simple biological samples was demonstrated using arachidonic acid as a model. Evidence was obtained suggesting that metal ions on the resin may be catalytic factors.