燃料微宇宙下底层水体中微生物群落活力与ATP生物负荷之间的关系。

The relationship between microbial community vitality and ATP bioburden in bottom waters under fuel microcosms.

作者信息

Passman Frederick Jay, Schmidt Jordan, Lewis Russell P

机构信息

Biodeterioration Control Associates, Inc., PO Box 3659, Princeton, NJ 08643-3659, USA.

LuminUltra Technologies, Ltd, 520 King Street, Fredericton, NB, E3B 6G3, Canada.

出版信息

Access Microbiol. 2023 Apr 26;5(4):acmi000411. doi: 10.1099/acmi.0.000411. eCollection 2023.

DOI:10.1099/acmi.0.000411

PMID:37223058

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10202405/

Abstract

Adenylate energy charge (AEC) - computed from the ATP, ADP and AMP concentrations in a specimen - reflect the net physiological state of the microbial population in that specimen. Previous research has demonstrated that healthy microbial populations maintain AEC≥0.8. As populations are subjected to stresses, or - in closed systems - deplete the available nutrients, respond to the accumulation of toxic metabolites, or both, AEC decreases (often to <0.5). Aqueous-phase samples from a set of fuel-water microcosms were tested for cellular ATP (cATP) and AEC. This paper reports on the precision of the AEC test method and the relationship between cellular AEC and cATP bioburdens in the aqueous phase of fuel over aqueous-phase microcosms.

摘要

腺苷酸能荷（AEC）——根据样本中ATP、ADP和AMP的浓度计算得出——反映了该样本中微生物群体的净生理状态。先前的研究表明，健康的微生物群体维持AEC≥0.8。当群体受到压力时，或者在封闭系统中耗尽可用营养物质、对有毒代谢产物的积累做出反应，或两者兼而有之时，AEC会降低（通常降至<0.5）。对一组燃料-水微观世界的水相样本进行了细胞ATP（cATP）和AEC测试。本文报告了AEC测试方法的精密度以及燃料水相微观世界水相中细胞AEC与cATP生物负荷之间的关系。