• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

高强度间歇训练对小鼠模型肩袖修复延迟后脂肪浸润的影响。

Effect of High-Intensity Interval Training on Fatty Infiltration After Delayed Rotator Cuff Repair in a Mouse Model.

作者信息

Zhou Hecheng, Wang Zili, Chen Chuanshun, Hu Hai, Jiang Binbin, Yin Yuesong, Zhang Kexiang, Shen Minren, Wu Song

机构信息

Department of Orthopaedic Surgery, The Third Xiangya Hospital of Central South University, Changsha, Hunan Province, China.

Clinical Medicine Eight-Year Program, Xiangya Medical School of Central South University, Changsha, Hunan, China.

出版信息

Orthop J Sports Med. 2023 May 19;11(5):23259671231170192. doi: 10.1177/23259671231170192. eCollection 2023 May.

DOI:10.1177/23259671231170192
PMID:37223073
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10201644/
Abstract

BACKGROUND

Fatty infiltration (FI) of the rotator cuff muscles is correlated with shoulder function and retear rates after rotator cuff repair. High-intensity interval training (HIIT) induces beige adipose tissue to express more uncoupling protein 1 (UCP1) to consume lipids. The beta-3 adrenergic receptor (β3AR) is located on adipocyte membrane and induces thermogenesis.

PURPOSE

To test the role of HIIT in improving muscle quality and contractility in a delayed rotator cuff repair mouse model via β3AR.

STUDY DESIGN

Controlled laboratory study.

METHODS

Three-month-old C57BL/6J mice underwent a unilateral supraspinatus (SS) tendon transection with a 6-week delayed tendon repair. Mice ran on a treadmill with the HIIT program for 6 weeks after tendon transection or after delayed repair. To study the role of β3AR, SR59230A, a selective β3AR antagonist, was administered to mice 10 minutes before each exercise through intraperitoneal injection. The SS, interscapular brown adipose tissue (iBAT), and subcutaneous inguinal white adipose tissue (ingWAT) were harvested at the end of the 12th week after tendon transection and were analyzed by histology and Western blotting. Tests were performed to assess muscle contractility of the SS.

RESULTS

Histologic analysis of SS showed that HIIT prevented and reversed muscle atrophy and FI. The contractile tests showed higher contractility of the SS in the HIIT groups than in the no-exercise group. In the HIIT groups, SS, iBAT, and ingWAT all showed increased expression of tyrosine hydroxylase, UCP1, and upregulated β3AR thermogenesis pathway. However, SR59230A inhibited HIIT, suggesting that the effect of HIIT depends on β3AR.

CONCLUSION

HIIT improved SS quality and function after delayed rotator cuff repair through a β3AR-dependent mechanism.

CLINICAL RELEVANCE

HIIT may serve as a new rehabilitation method for patients with rotator cuff muscle atrophy and FI after rotator cuff repair to improve postoperative clinical outcomes.

摘要

背景

肩袖肌群的脂肪浸润（FI）与肩袖修复后的肩部功能及再撕裂率相关。高强度间歇训练（HIIT）可诱导米色脂肪组织表达更多解偶联蛋白1（UCP1）以消耗脂质。β-3肾上腺素能受体（β3AR）位于脂肪细胞膜上并诱导产热。

目的

通过β3AR测试HIIT在延迟肩袖修复小鼠模型中改善肌肉质量和收缩力的作用。

研究设计

对照实验室研究。

方法

对3月龄C57BL/6J小鼠进行单侧冈上肌（SS）肌腱横断，并延迟6周进行肌腱修复。小鼠在肌腱横断后或延迟修复后按照HIIT方案在跑步机上跑步6周。为研究β3AR的作用，在每次运动前10分钟通过腹腔注射向小鼠给予选择性β3AR拮抗剂SR59230A。在肌腱横断后第12周结束时采集SS、肩胛间棕色脂肪组织（iBAT）和腹股沟皮下白色脂肪组织（ingWAT），并进行组织学和蛋白质印迹分析。进行测试以评估SS的肌肉收缩力。

结果

SS的组织学分析显示，HIIT可预防并逆转肌肉萎缩和FI。收缩力测试显示，HIIT组的SS收缩力高于无运动组。在HIIT组中，SS、iBAT和ingWAT均显示酪氨酸羟化酶、UCP1表达增加，且β3AR产热途径上调。然而，SR59230A抑制了HIIT，表明HIIT的作用依赖于β3AR。

结论

HIIT通过β3AR依赖性机制改善延迟肩袖修复后的SS质量和功能。

临床意义

HIIT可能作为一种新的康复方法，用于肩袖修复后肩袖肌群萎缩和FI的患者，以改善术后临床结局。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/43928444f9ad/10.1177_23259671231170192-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/340cc15a9582/10.1177_23259671231170192-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/607de7196b22/10.1177_23259671231170192-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/9c7b82846cfc/10.1177_23259671231170192-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/5d07bea41014/10.1177_23259671231170192-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/4f506feb2b53/10.1177_23259671231170192-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/4b73d5b33502/10.1177_23259671231170192-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/52ee86ca556e/10.1177_23259671231170192-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/99ebfb2beb36/10.1177_23259671231170192-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/76fa227384bb/10.1177_23259671231170192-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/43928444f9ad/10.1177_23259671231170192-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/340cc15a9582/10.1177_23259671231170192-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/607de7196b22/10.1177_23259671231170192-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/9c7b82846cfc/10.1177_23259671231170192-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/5d07bea41014/10.1177_23259671231170192-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/4f506feb2b53/10.1177_23259671231170192-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/4b73d5b33502/10.1177_23259671231170192-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/52ee86ca556e/10.1177_23259671231170192-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/99ebfb2beb36/10.1177_23259671231170192-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/76fa227384bb/10.1177_23259671231170192-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4257/10201644/43928444f9ad/10.1177_23259671231170192-fig10.jpg

相似文献

1
Effect of High-Intensity Interval Training on Fatty Infiltration After Delayed Rotator Cuff Repair in a Mouse Model.高强度间歇训练对小鼠模型肩袖修复延迟后脂肪浸润的影响。
Orthop J Sports Med. 2023 May 19;11(5):23259671231170192. doi: 10.1177/23259671231170192. eCollection 2023 May.
2
High-intensity interval training improves fatty infiltration in the rotator cuff through the β3 adrenergic receptor in mice.高强度间歇训练通过β3肾上腺素能受体改善小鼠肩袖的脂肪浸润。
Bone Joint Res. 2023 Aug 1;12(8):455-466. doi: 10.1302/2046-3758.128.BJR-2022-0309.R2.
3
Intramuscular Brown Fat Activation Decreases Muscle Atrophy and Fatty Infiltration and Improves Gait After Delayed Rotator Cuff Repair in Mice.肌肉内棕色脂肪激活可减少肌肉萎缩和脂肪浸润，并改善延迟肩袖修复后小鼠的步态。
Am J Sports Med. 2020 Jun;48(7):1590-1600. doi: 10.1177/0363546520910421. Epub 2020 Apr 13.
4
A Mouse Model of Delayed Rotator Cuff Repair Results in Persistent Muscle Atrophy and Fatty Infiltration.延迟肩袖修复的小鼠模型导致持续的肌肉萎缩和脂肪浸润。
Am J Sports Med. 2018 Oct;46(12):2981-2989. doi: 10.1177/0363546518793403. Epub 2018 Sep 10.
5
β-Adrenergic receptor agonist treats rotator cuff fatty infiltration by activating beige fat in mice.β-肾上腺素能受体激动剂通过激活小鼠的米色脂肪来治疗肩袖脂肪浸润。
J Shoulder Elbow Surg. 2021 Feb;30(2):373-386. doi: 10.1016/j.jse.2020.06.006. Epub 2020 Jun 27.
6
Overexpression of PRDM16 improves muscle function after rotator cuff tears.PRDM16 过表达可改善肩袖撕裂后的肌肉功能。
J Shoulder Elbow Surg. 2024 Dec;33(12):2725-2733. doi: 10.1016/j.jse.2024.05.037. Epub 2024 Jul 19.
7
Preoperative rotator cuff tendon integrity, tear size, and muscle atrophy and fatty infiltration are associated with structural outcomes of arthroscopic revision rotator cuff repair.术前肩袖肌腱完整性、撕裂大小、肌肉萎缩和脂肪浸润与关节镜下修复肩袖撕裂的结构结果相关。
Knee Surg Sports Traumatol Arthrosc. 2022 Jun;30(6):2029-2038. doi: 10.1007/s00167-021-06732-3. Epub 2021 Oct 12.
8
Beige fibro-adipogenic progenitor transplantation reduces muscle degeneration and improves function in a mouse model of delayed repair of rotator cuff tears. beige 纤维脂肪祖细胞移植减少了肌肉退化，改善了肩袖撕裂延迟修复小鼠模型的功能。
J Shoulder Elbow Surg. 2020 Apr;29(4):719-727. doi: 10.1016/j.jse.2019.09.021. Epub 2019 Nov 26.
9
Muscle-Derived Beige Adipose Precursors Secrete Promyogenic Exosomes That Treat Rotator Cuff Muscle Degeneration in Mice and Are Identified in Humans by Single-Cell RNA Sequencing.肌源性米色前体细胞分泌促肌生成外泌体，可治疗小鼠肩袖肌肉退化，并可通过单细胞 RNA 测序在人类中鉴定。
Am J Sports Med. 2022 Jul;50(8):2247-2257. doi: 10.1177/03635465221095568. Epub 2022 May 23.
10
Monitoring Rotator Cuff Muscle Fatty Infiltration Progression by Magnetic Resonance Imaging T1 Mapping: Correlation With Direct Evaluation Findings in Rats.通过磁共振成像 T1 映射监测肩袖肌肉脂肪浸润进展：与大鼠直接评估结果的相关性。
Am J Sports Med. 2022 Mar;50(4):1078-1087. doi: 10.1177/03635465211069976. Epub 2022 Jan 31.

引用本文的文献

1
Efficacy of hyaluronic acid and conditioned serum in acute rotator cuff tear repair: A rat model study.透明质酸和条件血清在急性肩袖撕裂修复中的疗效：一项大鼠模型研究。
Acta Orthop Traumatol Turc. 2025 Apr 29;59(2):86-92. doi: 10.5152/j.aott.2025.24020.

本文引用的文献

1
Resistance Exercise Training Improves Metabolic and Inflammatory Control in Adipose and Muscle Tissues in Mice Fed a High-Fat Diet.抗阻运动训练可改善高脂肪饮食喂养小鼠脂肪和肌肉组织的代谢和炎症控制。
Nutrients. 2022 May 24;14(11):2179. doi: 10.3390/nu14112179.
2
Muscle-Derived Beige Adipose Precursors Secrete Promyogenic Exosomes That Treat Rotator Cuff Muscle Degeneration in Mice and Are Identified in Humans by Single-Cell RNA Sequencing.肌源性米色前体细胞分泌促肌生成外泌体，可治疗小鼠肩袖肌肉退化，并可通过单细胞 RNA 测序在人类中鉴定。
Am J Sports Med. 2022 Jul;50(8):2247-2257. doi: 10.1177/03635465221095568. Epub 2022 May 23.
3
Adipose tissue-derived neurotrophic factor 3 regulates sympathetic innervation and thermogenesis in adipose tissue.
脂肪组织衍生神经营养因子 3 调节脂肪组织中的交感神经支配和产热。
Nat Commun. 2021 Sep 10;12(1):5362. doi: 10.1038/s41467-021-25766-2.
4
Retear rates after rotator cuff surgery: a systematic review and meta-analysis.肩袖手术后的再撕裂率：系统评价和荟萃分析。
BMC Musculoskelet Disord. 2021 Aug 31;22(1):749. doi: 10.1186/s12891-021-04634-6.
5
Sesamol promotes browning of white adipocytes to ameliorate obesity by inducing mitochondrial biogenesis and inhibition mitophagy via β3-AR/PKA signaling pathway.芝麻酚通过β3-肾上腺素能受体/蛋白激酶A信号通路诱导线粒体生物发生并抑制线粒体自噬，促进白色脂肪细胞褐变以改善肥胖。
Food Nutr Res. 2021 May 10;65. doi: 10.29219/fnr.v65.7577. eCollection 2021.
6
Exercise of high intensity ameliorates hepatic inflammation and the progression of NASH.高强度运动可改善肝脏炎症和 NASH 的进展。
Mol Metab. 2021 Nov;53:101270. doi: 10.1016/j.molmet.2021.101270. Epub 2021 Jun 10.
7
ADGRA1 negatively regulates energy expenditure and thermogenesis through both sympathetic nervous system and hypothalamus-pituitary-thyroid axis in male mice.ADGRA1 通过交感神经系统和下丘脑-垂体-甲状腺轴负调控雄性小鼠的能量消耗和产热。
Cell Death Dis. 2021 Apr 6;12(4):362. doi: 10.1038/s41419-021-03634-7.
8
microRNA-33 maintains adaptive thermogenesis via enhanced sympathetic nerve activity.miRNA-33 通过增强交感神经活性来维持适应性产热。
Nat Commun. 2021 Feb 16;12(1):843. doi: 10.1038/s41467-021-21107-5.
9
Effect of exercise on myosteatosis in adults: a systematic review and meta-analysis.运动对成年人肌内脂肪的影响：系统评价和荟萃分析。
J Appl Physiol (1985). 2021 Jan 1;130(1):245-255. doi: 10.1152/japplphysiol.00738.2020. Epub 2020 Nov 12.
10
Impact of combined training with different exercise intensities on inflammatory and lipid markers in type 2 diabetes: a secondary analysis from a 1-year randomized controlled trial.联合不同运动强度训练对 2 型糖尿病患者炎症和血脂标志物的影响：一项为期 1 年的随机对照试验的二次分析。
Cardiovasc Diabetol. 2020 Oct 7;19(1):169. doi: 10.1186/s12933-020-01136-y.