Selçuk University, Medical Faculty, Departments Physiology and Histology and Embriyology, Konya, Turkey.
Kirikkale University, Medical Faculty, Departments of Physiology, Kirikkale, Turkey.
J Trace Elem Med Biol. 2023 Sep;79:127217. doi: 10.1016/j.jtemb.2023.127217. Epub 2023 May 19.
Zinc, which is found in high concentrations in the β-cells of the pancreas, is also a critical component for the endocrine functions of the pancreas. SLC30A8/ZnT8 is the carrier protein responsible for the transport of zinc from the cytoplasm to the insulin granules. The aim of this study was to investigate how dietary zinc status affects pancreatic beta cell activation and ZnT8 levels in infant male rats born to zinc-deficient mothers.
The study was performed on male pups born to mothers fed a zinc-deficient diet. A total of 40 male rats were divided into 4 equal groups. Group 1: In addition to maternal zinc deficiency, this group was fed a zinc-deficient diet. Group 2: In addition to maternal zinc deficiency, this group was fed a standard diet. Group 3: In addition to maternal zinc deficiency, this group was fed a standard diet and received additional zinc supplementation. Group 4: Control group. Pancreas ZnT8 levels were determined by ELISA method and insulin-positive cell ratios in β-cells by immunohistochemistry.
The highest pancreatic ZnT8 levels and anti-insulin positive cell ratios in the current study were obtained in Group 3 and Group 4. In our study, the lowest pancreatic ZnT8 levels were obtained in Group 1 and Group 2, and the lowest pancreatic anti-insulin positive cell ratios were obtained in Group 1.
The results of the present study; in rats fed a zinc-deficient diet after maternal zinc deficiency has been established shows that ZnT8 levels and anti-insulin positive cell ratios in pancreatic tissue, which is significantly suppressed, reach control values with intraperitoneal zinc supplementation.
锌在胰腺的β细胞中浓度很高，也是胰腺内分泌功能的关键组成部分。SLC30A8/ZnT8 是负责将锌从细胞质转运到胰岛素颗粒的载体蛋白。本研究旨在探讨膳食锌状态如何影响缺锌母亲所生雄性幼鼠的胰腺β细胞激活和 ZnT8 水平。
本研究在缺锌饮食喂养的母亲所生雄性幼鼠中进行。总共 40 只雄性大鼠分为 4 组。第 1 组：除了母体缺锌外，该组还喂食缺锌饮食。第 2 组：除了母体缺锌外，该组还喂食标准饮食。第 3 组：除了母体缺锌外，该组还喂食标准饮食并接受额外的锌补充。第 4 组：对照组。通过 ELISA 法测定胰腺 ZnT8 水平，通过免疫组织化学法测定β细胞中胰岛素阳性细胞的比例。
本研究中胰腺 ZnT8 水平最高和β细胞中抗胰岛素阳性细胞比例最高的是第 3 组和第 4 组。在我们的研究中，第 1 组和第 2 组的胰腺 ZnT8 水平最低，第 1 组的胰腺抗胰岛素阳性细胞比例最低。
本研究结果表明，在母体缺锌后喂食缺锌饮食的大鼠中，胰腺组织中 ZnT8 水平和抗胰岛素阳性细胞比例显著受到抑制，通过腹腔内补锌可达到对照值。
