Salehi Ali, Naserzadeh Parvaneh, Tarighi Parastoo, Afjeh-Dana Elham, Akhshik Masoud, Jafari Amir, Mackvandi Pooyan, Ashtari Behnaz, Mozafari Masoud
Radiation Biology Research Centre, Iran University of Medical Sciences, Tehran, Iran; Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences. Tehran, Iran.
Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran.
Transl Oncol. 2023 Aug;34:101674. doi: 10.1016/j.tranon.2023.101674. Epub 2023 May 22.
Breast cancer is the most common cancer in women; it has been affecting the lives of millions each year globally and microfluidic devices seem to be a promising method for the future advancements in this field. This research uses a dynamic cell culture condition in a microfluidic concentration gradient device, helping us to assess breast anticancer activities of probiotic strains against MCF-7 cells. It has been shown that MCF-7 cells could grow and proliferate for at least 24 h; however, a specific concentration of probiotic supernatant could induce more cell death signaling population after 48 h. One of our key findings was that our evaluated optimum dose (7.8 mg/L) was less than the conventional static cell culture treatment dose (12 mg/L). To determine the most effective dose over time and the percentage of apoptosis versus necrosis, flowcytometric assessment was performed. Exposing the MCF-7 cells to probiotic supernatant after 6, 24 and 48 h, confirmed that the apoptotic and necrotic cell death signaling were concentration and time dependent. We have shown a case that these types of microfluidics platforms performing dynamic cell culture could be beneficial in personalized medicine and cancer therapy.
乳腺癌是女性中最常见的癌症；每年在全球范围内影响着数百万人的生活，而微流控设备似乎是该领域未来发展的一种有前景的方法。本研究在微流控浓度梯度装置中采用动态细胞培养条件，帮助我们评估益生菌菌株对MCF - 7细胞的乳腺癌抗癌活性。结果表明，MCF - 7细胞可以生长和增殖至少24小时；然而，特定浓度的益生菌上清液在48小时后可诱导更多细胞死亡信号群体。我们的一项关键发现是，我们评估的最佳剂量（7.8毫克/升）低于传统静态细胞培养治疗剂量（12毫克/升）。为了确定随时间变化的最有效剂量以及凋亡与坏死的百分比，进行了流式细胞术评估。在6小时、24小时和48小时后将MCF - 7细胞暴露于益生菌上清液，证实凋亡和坏死性细胞死亡信号是浓度和时间依赖性的。我们展示了一个案例，即这些进行动态细胞培养的微流控平台在个性化医学和癌症治疗中可能是有益的。
