School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia.
School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia.
J Glob Antimicrob Resist. 2023 Jun;33:337-344. doi: 10.1016/j.jgar.2023.05.003. Epub 2023 May 22.
The aim of this study was to characterise an early clinical multiply antibiotic resistant Acinetobacter baumannii global clone 1 (GC1) isolate from Africa.
The draft genome sequence was determined using short-read (Illumina MiSeq) sequence data and compared to other early GC1 isolates. Resistance genes and other features were identified using various bioinformatics tools. Plasmids were visualised.
LUH6050, recovered in South Africa between January 1997 and January 1999, is ST1:ST231:KL1:OCL1. Several antibiotic resistance genes (aacC1, aadA2, aphA1, catA1, sul1, and tetA(A)) reside in AbaR32. LUH6050 also includes the plasmid pRAY*, carrying the aadB gentamicin and tobramycin resistance gene, and a 29.9 kb plasmid, pLUH6050-3, carrying the msrE-mphE (macrolide resistance) and dfrA44 (trimethoprim resistance) genes and a small cryptic Rep_1 plasmid. Plasmid pLUH6050-3, a cointegrate of pA1-1 (R3-T1; RepAci1) with an R3-T33 type plasmid encoding a different Rep_3 family Rep, carries 15 pdif sites and 13 dif modules, including those that carry the mrsE-mphE and dfrA44 genes and three that include toxin-antitoxin gene pairs. The closest relative of pLUH6050-3 found in GenBank was from an unrelated 2013 Tanzanian A. baumannii isolate. The chromosome has an AbaR0-type region in comM and includes no ISAba1 copies. Similar features were found in most other sequenced lineage 1 GC1 isolates recovered prior to 2000.
LUH6050 represents an early form of the GC1 lineage 1, supplementing limited information about early isolates and isolates from Africa. These data contribute to the understanding of the emergence, evolution, and dissemination of the A. baumannii GC1 clonal complex.
本研究旨在对来自非洲的一株早期临床多重耐药鲍曼不动杆菌全球克隆 1（GC1）分离株进行特征描述。
使用短读长（Illumina MiSeq）序列数据测定该分离株的基因组草图序列，并与其他早期 GC1 分离株进行比较。使用各种生物信息学工具来鉴定耐药基因和其他特征。可视化质粒。
LUH6050 于 1997 年 1 月至 1999 年 1 月期间在南非被分离，其 ST 型为 ST1:ST231:KL1:OCL1。几种抗生素耐药基因（aacC1、aadA2、aphA1、catA1、sul1 和 tetA(A))位于 AbaR32 中。LUH6050 还包含携带 aadB 庆大霉素和妥布霉素耐药基因的质粒 pRAY*，以及一个 29.9kb 的质粒 pLUH6050-3，其携带 msrE-mphE（大环内酯类耐药）和 dfrA44（甲氧苄啶耐药）基因以及一个小型隐匿 Rep_1 质粒。质粒 pLUH6050-3 是一个由 pA1-1（R3-T1；RepAci1）和一个编码不同 Rep_3 家族 Rep 的 R3-T33 型质粒构成的嵌合体，带有 15 个 pdif 位点和 13 个 dif 模块，其中包括携带 mrsE-mphE 和 dfrA44 基因的模块以及 3 个包含毒素-抗毒素基因对的模块。在 GenBank 中发现的与 pLUH6050-3 最接近的基因是来自 2013 年坦桑尼亚一株不相关的鲍曼不动杆菌分离株。染色体在 comM 中具有 AbaR0 型区域，不包含 ISAba1 拷贝。在大多数其他在 2000 年前分离的 1 型谱系 1 GC1 分离株中发现了类似的特征。
LUH6050 代表了 GC1 谱系 1 的早期形式，补充了关于早期分离株和来自非洲的分离株的有限信息。这些数据有助于了解鲍曼不动杆菌 GC1 克隆复合体的出现、进化和传播。
