Silva A G, Huber J T, Herdt T H, Holland R, Degregorio R M, Mullaney T P
J Dairy Sci. 1986 May;69(5):1387-93. doi: 10.3168/jds.S0022-0302(86)80545-8.
Sixteen male Holstein calves were fed milk replacer (14% solids) containing 23% crude protein from: 100% milk protein; or 66% soybean protein concentrate plus 34% milk protein as the only nutrients at 8, 9, 10, 11, 12, 12, and 12% body weight from 1 to 7 wk of age, respectively. Eight calves were sensitized to soybean by feeding 66% soybean protein concentrate plus 34% milk protein for 21 d and eight were not sensitized by feeding 100% milk protein. Afterward, each calf received one of the diets for 10 d followed by the other diet for an additional 10 d. During the 2nd wk of the initial period each calf was surgically fitted with a duodenal cannula for biopsy of intestinal mucosa. Biopsies were taken at surgery and on the last day of each period. Feeding 66% soybean protein concentrate plus 34% milk protein resulted in lower body weight gain, decreased feed efficiency, higher rectal temperatures, increased diarrhea, and villus atrophy. Diminished villi size supports other studies reporting allergic reaction to soybean protein and was associated with the poor performance observed in calves fed soybean protein.
16头雄性荷斯坦犊牛被饲喂含23%粗蛋白的代乳粉（14%固形物），代乳粉来源如下：100%乳蛋白；或66%大豆浓缩蛋白加34%乳蛋白，分别从1至7周龄按体重的8%、9%、10%、11%、12%、12%和12%进行投喂，作为唯一营养源。8头犊牛通过饲喂66%大豆浓缩蛋白加34%乳蛋白持续21天对大豆产生致敏，另外8头通过饲喂100%乳蛋白未产生致敏。之后，每头犊牛先接受一种日粮10天，接着再接受另一种日粮10天。在初始期的第2周，每头犊牛通过手术安装十二指肠套管以便对肠黏膜进行活检。在手术时以及每个阶段的最后一天进行活检。饲喂66%大豆浓缩蛋白加34%乳蛋白导致体重增加降低、饲料效率下降、直肠温度升高、腹泻增加以及绒毛萎缩。绒毛尺寸减小支持了其他关于对大豆蛋白过敏反应的研究报告，并且与饲喂大豆蛋白的犊牛中观察到的生长性能不佳有关。
