经皮附睾精子抽吸术对小鼠精子质量的影响。

The impact of percutaneous epididymal sperm aspiration on sperm quality in mice.

机构信息

Institute of in vivo and in vitro Models, University of Veterinary Medicine Vienna, Vienna, Austria.

Department for Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria.

出版信息

Reprod Fertil. 2023 Jun 19;4(2). doi: 10.1530/RAF-23-0017. Print 2023 Apr 1.

DOI:10.1530/RAF-23-0017

PMID:37227214

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10305637/

Abstract

ABSTRACT

In laboratory mice, sperm quality is usually assessed in spermatozoa collected from the cauda epididymidis of freshly sacrificed males. Percutaneous epididymal sperm aspiration (PESA) is a non-terminal alternative that would allow repeated sperm collection for sperm quality assessment in living males. To test whether PESA is a suitable method to assess sperm quality, we compared sperm traits between samples collected by PESA vs the commonly applied terminal cauda epididymidis dissection. The collected sperm samples were analyzed using computer-assisted sperm analysis and various parameters, including sperm motility, swimming velocity and morphology, were determined. We were able to retrieve motile sperm from all mice using PESA and the terminal cauda epididymidis dissection. Based on computer-assisted sperm analysis, however, sperm motility and swimming velocity were significantly lower after PESA compared to samples obtained by cauda epididymidis dissection. In addition, we found significantly more morphological abnormalities in PESA samples, probably induced as a side effect of the sampling technique. Although sperm samples collected by PESA are successfully used for in vitro fertilization, we cannot recommend PESA as a suitable method to assess sperm quality in mice, since the procedure seems to impair various sperm traits.

LAY SUMMARY

In mice, sperm quality is usually assessed in sperm collected from the epididymis (organ where ripe sperm is stored) of euthanized males. However, there is one non-terminal and minimal invasive alternative to collect sperm, called percutaneous epididymal sperm aspiration (PESA), which allows repeated sample collections from the same individual. Given that individual sperm quality is variable and can change according to various factors, PESA could allow to track sperm quality over time and would be highly appreciated in different research fields. Here, we tested the suitability of PESA to determine sperm quality by comparing sperm samples collected by PESA vs the commonly applied terminal epididymis dissection. We used computer-assisted sperm analysis to determine various sperm quality traits. Surprisingly, we found that sperm collected by PESA showed significantly reduced motility, swimming velocity and more morphological abnormalities compared to sperm samples collected by epididymis dissection. Thus, we cannot recommend PESA as a suitable method to determine sperm quality traits as the procedure itself seems to affect collected sperm cells.

摘要

在实验小鼠中，通常通过从刚处死的雄性动物的附睾尾部收集精子来评估精子质量。经皮附睾精子抽吸术（PESA）是一种非终末选择，可允许在活体雄性动物中重复进行精子收集以评估精子质量。为了测试 PESA 是否是评估精子质量的合适方法，我们比较了通过 PESA 收集的精子样本与常用的末端附睾尾部解剖收集的精子样本之间的精子特征。使用计算机辅助精子分析（CASA）分析收集的精子样本，并确定了各种参数，包括精子活力、泳动速度和形态。我们能够通过 PESA 从所有小鼠中检索到有活力的精子，并且通过末端附睾尾部解剖也可以获得有活力的精子。然而，根据 CASA 分析，与通过附睾尾部解剖获得的样本相比，PESA 后精子活力和泳动速度明显较低。此外，我们发现 PESA 样本中存在更多的形态异常，这可能是由于采样技术的副作用所致。尽管通过 PESA 收集的精子样本可成功用于体外受精，但我们不能推荐 PESA 作为评估小鼠精子质量的合适方法，因为该程序似乎会损害各种精子特征。

简介

在小鼠中，通常通过从处死雄性动物的附睾（储存成熟精子的器官）中收集的精子来评估精子质量。然而，有一种非终末和微创的替代方法可以收集精子，称为经皮附睾精子抽吸术（PESA），它允许从同一个体中重复进行样本采集。鉴于个体精子质量是可变的，并且可以根据各种因素而变化，PESA 可以允许随时间跟踪精子质量，并且在不同的研究领域中会受到高度赞赏。在这里，我们通过比较通过 PESA 收集的精子样本与常用的末端附睾解剖收集的精子样本，测试了 PESA 确定精子质量的适用性。我们使用计算机辅助精子分析来确定各种精子质量特征。令人惊讶的是，我们发现与通过附睾解剖收集的精子样本相比，通过 PESA 收集的精子显示出明显降低的活力、泳动速度和更多的形态异常。因此，我们不能推荐 PESA 作为确定精子质量特征的合适方法，因为该过程本身似乎会影响收集的精子细胞。