通过免疫亲和色谱法从人红细胞中纯化铜锌超氧化物歧化酶。存在等电异质性的证据。
Purification of Cu-Zn-superoxide dismutase from human erythrocytes by immunoaffinity chromatography. Evidence for the presence of isoelectric heterogeneity.
作者信息
Arai K, Iizuka S, Makita A, Oikawa K, Taniguchi N
出版信息
J Immunol Methods. 1986 Jul 11;91(1):139-43. doi: 10.1016/0022-1759(86)90112-2.
PMID:3722829
Abstract
Cu-Zn-superoxide dismutase (SOD) was prepared in highly purified form from human erythrocytes by immunoaffinity chromatography using anti-human Cu-Zn-SOD goat IgG. The purified SOD had a high specific activity and gave a single band on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. On isoelectric focusing and immunoblotting several bands with enzyme activity and antigenicity were discerned.
摘要
采用抗人铜锌超氧化物歧化酶（Cu-Zn-SOD）山羊IgG通过免疫亲和层析从人红细胞中制备出高纯度的铜锌超氧化物歧化酶（SOD）。纯化后的SOD具有较高的比活性，在十二烷基硫酸钠存在的情况下，在聚丙烯酰胺凝胶电泳上呈现单一谱带。在等电聚焦和免疫印迹中，可识别出几条具有酶活性和抗原性的谱带。
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