Vexler A M, Litinskaya L L
Int J Hyperthermia. 1986 Jan-Mar;2(1):75-81. doi: 10.3109/02656738609019996.
The effect of hyperthermia on pH of cells cultivated at varying pH of the medium and under different conditions of oxygenation has been studied. Chinese hamster fibroblasts and pig embryo kidney cells were used. The intracellular pH (pHi) was determined by the microfluorimetric method using fluorescein diacetate. The required temperature (37 degrees C and 41 degrees C) was maintained by a warm stage in combination with a thermochamber. Different conditions of oxygenation were achieved by placing the cells into an open (euoxic) or closed (hypoxic) chamber. Both hyperthermia and hypoxia are shown to result in the acidification of cells. The effect increases with the duration of exposure to the agents used. The decrease of pHi is more pronounced when hyperthermia is combined with hypoxia or when the pH of the medium is low. The results obtained correlate with the magnitude of directly damaging and radiosensitizing effects of hyperthermia being dependent on the environmental conditions.
研究了高温对在不同pH值培养基中培养且处于不同氧合条件下的细胞pH值的影响。使用了中国仓鼠成纤维细胞和猪胚胎肾细胞。通过使用荧光素二乙酸酯的微荧光法测定细胞内pH值（pHi）。通过暖台结合热室维持所需温度（37℃和41℃）。将细胞置于开放（常氧）或封闭（低氧）培养箱中可实现不同的氧合条件。结果表明，高温和低氧均会导致细胞酸化。随着暴露于所用试剂时间的延长，这种影响会增强。当高温与低氧联合或培养基pH值较低时，pHi的降低更为明显。所得结果与高温的直接损伤和放射增敏作用的程度相关，且该作用取决于环境条件。
