van Kuijk F J, Thomas D W, Konopelski J P, Dratz E A
J Lipid Res. 1986 Apr;27(4):452-6.
A novel method is presented for transesterification of fatty acid esters in phospholipids and triglycerides to benzyl esters while simultaneously recovering free fatty acids as methyl esters. Transesterification is catalyzed by 0.2 M (m-trifluoromethyl phenyl)trimethyl ammonium hydroxide in methylene chloride, 10% (v/v) benzyl alcohol, and 1% (w/v) potassium tert-butoxide, and is complete in 30 min at room temperature. Methyl esters of all common fatty acids separate from the benzyl esters formed from phospholipids. This method has broad utility and is applicable to the formation of esters optimized for detection by absorbance or fluorescence (high performance liquid chromatography), electron capture (gas-liquid chromatography), or negative ion chemical ionization (gas-liquid chromatography-mass spectrometry).
提出了一种新方法，可将磷脂和甘油三酯中的脂肪酸酯酯交换为苄酯，同时将游离脂肪酸回收为甲酯。酯交换反应由0.2 M（间三氟甲基苯基）三甲基氢氧化铵在二氯甲烷、10%（v/v）苄醇和1%（w/v）叔丁醇钾中催化，在室温下30分钟内完成。所有常见脂肪酸的甲酯与由磷脂形成的苄酯分离。该方法具有广泛的用途，适用于形成经优化可通过吸光度或荧光（高效液相色谱）、电子捕获（气液色谱）或负离子化学电离（气液色谱-质谱）进行检测的酯。
