Tsin A T, Flores J M
J Exp Biol. 1986 May;122:269-75. doi: 10.1242/jeb.122.1.269.
Goldfish with retinas rich in either rhodopsin or porphyropsin were illuminated with bright light and then placed in the dark room to allow visual pigment regeneration. The kinetics of this in vivo pigment regeneration were followed by sampling these animals at regular time intervals. The first-order kinetic rate constant for the initial period of porphyropsin regeneration at 20 degrees C was 8.3 X 10(-3) nmol kg-1 body weight min-1 and the half-life of this reaction was 83 min. At 30 degrees C, the rate constant was increased to 1.4 X 10(-2) nmol kg-1 body weight min-1, yielding a reduced half-life of 49 min. This suggests that the Q10 of porphyropsin regeneration is about 1.7. In goldfish retinas enriched with rhodopsin (62% rhodopsin and 38% porphyropsin), the initial phase of visual pigment regeneration (at 30 degrees C) proceeded at a slower rate (first-order rate constant: 6.5 X 10(-3) nmol kg-1 body weight min-1; half-life of reaction = 106 min) than the rate of porphyropsin regeneration. This suggests that the high proportion of rhodopsin in the retina of goldfish held at 30 degrees C is not a direct result of a faster rate of regeneration of rhodopsin than of porphyropsin.
将富含视紫红质或视紫蓝质的金鱼置于强光下照射，然后放入暗室中以实现视觉色素再生。通过定期对这些动物进行取样，跟踪这种体内色素再生的动力学过程。在20摄氏度时，视紫蓝质再生初始阶段的一级动力学速率常数为8.3×10⁻³纳摩尔·千克⁻¹体重·分钟⁻¹，该反应的半衰期为83分钟。在30摄氏度时，速率常数增加到1.4×10⁻²纳摩尔·千克⁻¹体重·分钟⁻¹，半衰期缩短至49分钟。这表明视紫蓝质再生的温度系数Q10约为1.7。在富含视紫红质的金鱼视网膜中（视紫红质占62%，视紫蓝质占38%），视觉色素再生的初始阶段（在30摄氏度时）进行得比视紫蓝质再生的速率要慢（一级速率常数：6.5×10⁻³纳摩尔·千克⁻¹体重·分钟⁻¹；反应半衰期 = 106分钟）。这表明在30摄氏度下饲养的金鱼视网膜中视紫红质的高比例并非视紫红质再生速率比视紫蓝质更快的直接结果。
