Flanagan R J, Charleson F P, Synnes E I, Wiebe L I
J Nucl Med. 1986 Jul;27(7):1165-71.
Iodine-125 (125I) and iodine-131- (131I) 6-iodocholest-5-en-3 beta-ol has been prepared directly from 6-chloromercuricholest-5-en-3 beta-ol and [125I] or [131I]sodium iodide. This method produces material of "no-carrier-added" specific activity and excellent radiochemical purity. The entire procedure is complete in 10 min and can be carried out in 95% ethanol. The biodistribution of this new high specific activity form of [131I]-6-iodocholest-5-en-3 beta-ol has been measured in rats and found to be very similar to that found for low specific activity [131I]-6-iodocholest-5-en-3 beta-ol produced by exchange labeling. The whole-body elimination curve over a 4-day period was measured and a dependence between the rate of elimination and specific activity was detected. Products of three different specific activities in addition to "no-carrier-added" material were studied.
碘 - 125（¹²⁵I）和碘 - 131（¹³¹I）标记的6 - 碘胆甾 - 5 - 烯 - 3β - 醇已由6 - 氯汞胆甾 - 5 - 烯 - 3β - 醇与[¹²⁵I]或[¹³¹I]碘化钠直接制备。该方法产生“无载体添加”比活度的物质且放射化学纯度极高。整个过程在10分钟内完成，且可在95%乙醇中进行。已在大鼠中测定了这种新的高比活度形式的[¹³¹I] - 6 - 碘胆甾 - 5 - 烯 - 3β - 醇的生物分布，发现其与通过交换标记产生的低比活度[¹³¹I] - 6 - 碘胆甾 - 5 - 烯 - 3β - 醇的生物分布非常相似。测量了4天期间的全身消除曲线，并检测到消除速率与比活度之间的相关性。除了“无载体添加”物质外，还研究了三种不同比活度的产物。
