Institute of Clinical Biochemistry and Diagnostics, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czechia.
Department of Neurology, Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Czechia.
Pharmacology. 2023;108(4):359-367. doi: 10.1159/000529970. Epub 2023 May 22.
Our study aimed to investigate the effect of zonisamide (ZNS) on bone metabolism in the rat model.
Eight-week-old rats were divided into four groups. The sham-operated control group (SHAM) and the control group after orchidectomy (ORX) received the standard laboratory diet (SLD). The experimental group after orchidectomy (ORX+ZNS) and the sham-operated control group (SHAM+ZNS) received SLD enriched with ZNS for 12 weeks. Bone marker concentrations in serum of receptor activator of nuclear factor kappa B ligand, PINP, and osteoprotegerin, and the levels of sclerostin and bone alkaline phosphatase in bone homogenate, were measured using an enzyme-linked immunosorbent assay. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry. The femurs were used for biomechanical testing.
We found a statistically significant reduction in BMD and biomechanical strength 12 weeks after orchidectomy of the rats (ORX). After ZNS administration to orchidectomized rats (ORX+ZNS) and the sham-operated control rats (SHAM+ZNS), there were no statistically significant changes in BMD, bone turnover markers, or biomechanical properties as compared with the ORX group and SHAM group.
The results suggest that administration of ZNS to rats exerts no negative effect on BMD, bone metabolism markers, or biomechanical properties.
本研究旨在探讨佐米曲普坦（ZNS）对大鼠模型骨代谢的影响。
将 8 周龄大鼠分为四组。假手术对照组（SHAM）和去势对照组（ORX）给予标准实验室饮食（SLD）。去势实验组（ORX+ZNS）和假手术对照组（SHAM+ZNS）给予 SLD 中添加 ZNS 的饮食 12 周。采用酶联免疫吸附试验检测血清中核因子κB 受体激活剂配体（RANKL）、PINP 和骨保护素的浓度，以及骨匀浆中骨硬化素和骨碱性磷酸酶的水平。采用双能 X 射线吸收法测量骨密度（BMD）。使用生物力学试验机对股骨进行测试。
我们发现，去势后 12 周大鼠的 BMD 和生物力学强度显著降低（ORX）。去势大鼠（ORX+ZNS）和假手术对照组大鼠（SHAM+ZNS）给予 ZNS 后，与 ORX 组和 SHAM 组相比，BMD、骨转换标志物或生物力学特性均无统计学显著变化。
结果表明，ZNS 给药对大鼠的 BMD、骨代谢标志物或生物力学特性没有不良影响。
