Department of Medical Parasitology, Faculty of Medicine, Menoufia University, Shebin al-Kom, Menoufia, 6132720, Egypt.
Department of Pathology, Faculty of Medicine, Menoufia University, Shebin al-Kom, Menoufia, 6132720, Egypt.
J Helminthol. 2023 May 26;97:e44. doi: 10.1017/S0022149X23000214.
Trichinosis is a global food-borne zoonotic disease. Most drugs used in its treatment have low bioavailability and reduced activity against larvae. Therefore, there is an urgent need for safe and effective medications. This study aimed to investigate the anti-parasitic and anti-inflammatory efficacy of olibanum (OL) extract, alone or combined with albendazole (ABZ) during both intestinal and muscular phases of trichinosis. Male Swiss albino mice (n = 130) were allocated to seven groups, with 20 mice in each group except for the negative control group (10 mice): negative control (GI), positive control (GII), OL25- treated (GIII), OL50- treated (GIV), ABZ50- treated (GV), OL25 + ABZ25 (GVI), and OL50 + ABZ25 (GVII). For intestinal and muscular phase analysis, each group was divided into two subgroups based on euthanizing day (6 and 35 days post-infection). The drug's efficacy was evaluated through parasitological, biochemical, histopathological, and immunohistochemical studies. OL extract at both concentrations (25 mg/kg/d, 50 mg/kg/d) significantly reduced adult (53.7% and 68.1%, respectively) and larval counts (57.3% and 78.8%, respectively). It improved the histopathological changes in intestine and muscle. The expression of CD8 T cells and the serum level of IL-10 increased significantly during both intestinal and muscular phases ( < 0.05) in OL50 treated mice. Additionally, OL decreased abnormal levels of liver enzymes (ALT & AST). Its effects were dose-dependent in both adult and larval stages. In conclusion, OL exhibits promising activity against both stages of infection, particularly at the intramuscular phase. It can be safe as an alternative treatment for trichinosis.
旋毛虫病是一种全球性的食源性人畜共患病。大多数用于治疗的药物生物利用度低，对幼虫的活性降低。因此，迫切需要安全有效的药物。本研究旨在研究乳香（OL）提取物单独或与阿苯达唑（ABZ）联合在旋毛虫病的肠道和肌肉期的抗寄生虫和抗炎疗效。雄性瑞士白化病小鼠（n = 130）分为七组，每组 20 只，除阴性对照组（10 只）外：阴性对照组（GI）、阳性对照组（GII）、OL25 治疗组（GIII）、OL50 治疗组（GIV）、ABZ50 治疗组（GV）、OL25 + ABZ25 治疗组（GVI）和 OL50 + ABZ25 治疗组（GVII）。对于肠道和肌肉期分析，根据安乐死日（感染后 6 天和 35 天）将每组分为两个亚组。通过寄生虫学、生化、组织病理学和免疫组织化学研究评估药物的疗效。OL 提取物在两个浓度（25 mg/kg/d，50 mg/kg/d）下显著降低了成虫（分别为 53.7%和 68.1%）和幼虫计数（分别为 57.3%和 78.8%）。它改善了肠和肌肉的组织病理学变化。在 OL50 治疗的小鼠中，CD8 T 细胞的表达和血清中 IL-10 的水平在肠道和肌肉期均显著增加（<0.05）。此外，OL 降低了异常的肝酶（ALT 和 AST）水平。它在成虫和幼虫阶段均表现出剂量依赖性的作用。总之，OL 对感染的两个阶段均表现出有希望的活性，特别是在肌肉期。它可以作为旋毛虫病的替代治疗方法是安全的。
