Boler Michael, Anderson Mark, Rodgers Mary, Parumoottil Jessica, Olivo Ana, Harris Barbara, Stec Michael, Gosha Amy, Behun Dylan, Holzmayer Vera, Anderson Abby, Greenholt Ella, Fortney Tiffany, Almaraz Eduardo, Cloherty Gavin, Landay Alan, Moy James
Rush University Medical Center, 1725 W Harrison Street Suite 739, Chicago, IL 60612, USA.
Abbott Laboratories, 100 Abbott Park Rd, Abbott Park, IL 60064, USA.
IJID Reg. 2023 Jun;7:277-280. doi: 10.1016/j.ijregi.2023.04.014. Epub 2023 May 12.
Commercial severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody tests were developed before variants with spike protein mutations emerged, leading to concerns that these tests have reduced sensitivity for detecting antibody responses in individuals infected with Omicron subvariants. This study was performed to evaluate Abbott ARCHITECT serologic assays, AdviseDx SARS-CoV-2 IgG II, and SARS-CoV-2 IgG for the detection of spike (S) and nucleocapsid (N) IgG antibody increases in vaccinated healthcare workers infected with Omicron subvariants.
During the BA.1/2 and BA.4/5 waves, 171 SARS-CoV-2-infected individuals (122 in the BA.1/2 wave, 49 in the BA.4/5 wave) were tested for S and N IgG post infection. Sequencing and SARS-CoV-2 variant confirmation were performed on nasal swab samples from individuals infected during the BA.1/2 wave.
Twenty-seven Omicron sequence confirmed individuals in the BA.1/2 wave and all 49 in the BA.4/5 wave had pre-infection antibody data. Compared to pre-infection levels, post-infection S IgG increased 6.6-fold from 1294 ± 302 BAU/ml (mean ± standard error measurement) to 9796 ± 1252 BAU/ml ( < 0.001) during the BA.1/2 wave, and 3.6-fold from 1771 ± 351 BAU/ml to 8224 ± 943 BAU/ml ( < 0.001) during the BA.4/5 wave. N IgG increased post infection 19.1-fold from 0.2 ± 0.1 to 3.7 ± 0.5 ( < 0.001) during the BA.1/2 wave and 13.5-fold from 0.22 ± 0.1 to 3.2 ± 0.3 ( < 0.001) during the BA.4/5 wave. Among 159 infection-naïve individuals, positive N IgG levels were detected with a sensitivity of 88% in the 87 individuals who were tested between 14 days and 60 days post infection.
The large increases in post-infection S IgG along with the N IgG sensitivity that was comparable to previously reported N IgG sensitivity data in unvaccinated individuals after Omicron infection, support the use of Abbott SARS-CoV-2 assays for detecting increased S IgG and seroconversion of N IgG in vaccinated individuals post Omicron infection. Given that 68% of the United States population is fully vaccinated, these results are of current relevance.
商业性严重急性呼吸综合征冠状病毒2（SARS-CoV-2）抗体检测是在出现刺突蛋白突变的变体之前开发的，这引发了人们对这些检测在检测感染奥密克戎亚变体个体的抗体反应时敏感性降低的担忧。本研究旨在评估雅培ARCHITECT血清学检测、AdviseDx SARS-CoV-2 IgG II和SARS-CoV-2 IgG检测接种疫苗的医护人员感染奥密克戎亚变体后刺突（S）和核衣壳（N）IgG抗体增加情况。
在BA.1/2和BA.4/5疫情波期间，对171名SARS-CoV-2感染个体（BA.1/2疫情波中有122名，BA.4/5疫情波中有49名）在感染后进行S和N IgG检测。对BA.1/2疫情波期间感染个体的鼻拭子样本进行测序和SARS-CoV-2变体确认。
BA.1/2疫情波中有27名奥密克戎序列确认个体，BA.4/5疫情波中的所有49名个体均有感染前抗体数据。与感染前水平相比，感染后S IgG在BA.1/2疫情波期间从1294±302 BAU/ml（平均值±标准误差）增加6.6倍至9796±1252 BAU/ml（P<0.001），在BA.4/5疫情波期间从1771±351 BAU/ml增加3.6倍至8224±943 BAU/ml（P<0.001）。N IgG在BA.1/2疫情波期间感染后从0.2±0.1增加至至3.7±0.5，增加19.1倍（P<0.001），在BA.4/5疫情波期间从0.22±0.1增加至3.2±0.3，增加13.5倍（P<0.001）。在159名未感染过的个体中，在感染后14天至60天之间接受检测的87名个体中，N IgG阳性水平的检测灵敏度为88%。
感染后S IgG大幅增加，以及N IgG的灵敏度与先前报道的奥密克戎感染后未接种疫苗个体的N IgG灵敏度数据相当，支持使用雅培SARS-CoV-2检测来检测接种疫苗个体感染奥密克戎后S IgG增加和N IgG血清转化。鉴于美国68%的人口已完全接种疫苗，这些结果具有当前相关性。
