Shanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan 030006, China.
Department of Biology, Georgia Southern University, Statesboro GA 30460-8042, USA.
Ying Yong Sheng Tai Xue Bao. 2023 May;34(5):1395-1403. doi: 10.13287/j.1001-9332.202305.031.
To reveal the assembly mechanisms of soil protozoan community in subalpine forest ecosystems, we analyzed the composition and diversity of protozoan communities and their drivers at the six strata (the litter profile, humus profile, 0-10 cm, 10-20 cm, 20-40 cm and 40-80 cm) of soil profiles in subalpine forest in Luya Mountain using Illumina Miseq high-throughput sequencing technology. The results showed that protozoa in the soil profiles belonged to 335 genera, 206 families, 114 orders, 57 classes, 21 phyla, and 8 kingdoms. There were five dominant phyla (relative abundance >1%) and 10 dominant families (relative abundance >5%). The α diversity decreased significantly with increasing soil depth. Results of PCoA analysis showed that the spatial composition and structure of protozoan community differed significantly across soil depths. The results of RDA analysis showed that soil pH and soil water content were important factors driving protozoan community structure across soil profile. Null model analysis suggested that the heterogeneous selection dominated the processes of protozoan community assemblage. Molecular ecological network analysis revealed that the complexity of soil proto-zoan communities decreased continuously with increasing depth. These results elucidate the assembly mechanism of soil microbial community in subalpine forest ecosystem.
为了揭示亚高山森林生态系统中土壤原生动物群落的组装机制，我们利用 Illumina Miseq 高通量测序技术，分析了亚高山森林土壤剖面 6 个层次（凋落物层、腐殖质层、0-10cm、10-20cm、20-40cm 和 40-80cm）中原生动物群落的组成和多样性及其驱动因素。结果表明，土壤剖层中的原生动物属于 335 属、206 科、114 目、57 纲、21 门和 8 界。有五个主要的门（相对丰度>1%）和十个主要的科（相对丰度>5%）。α多样性随土壤深度的增加而显著降低。PCoA 分析结果表明，原生动物群落的空间组成和结构在不同土壤深度上有显著差异。RDA 分析结果表明，土壤 pH 值和土壤含水量是驱动土壤剖面中原生动物群落结构的重要因素。零模型分析表明，异质选择主导了原生动物群落组装的过程。分子生态网络分析表明，土壤原生动物群落的复杂性随深度的增加而连续下降。这些结果阐明了亚高山森林生态系统中土壤微生物群落的组装机制。
