Han Ning, Li Jie, Wan Peng, Pan Yu, Xu Tiantian, Xiong Wenguang, Zeng Zhenling
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
National Laboratory of Safety Evaluation (Environmental Assessment) of Veterinary Drugs, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Antibiotics (Basel). 2023 Apr 28;12(5):825. doi: 10.3390/antibiotics12050825.
This study was performed to investigate the presence and characteristics of the oxazolidinone resistance genes and (D) in . In total, 36 isolates (30 isolates, 6 isolates) were collected from pig farms in China in 2020-2021, using PCR to determine the presence of and . Then, 2 of the 36 Streptococcus isolates were further processed as follows. Whole-genome sequencing and de novo assembly were employed to analyze the genetic environment of the and (D) genes. Conjugation and inverse PCR were employed to verify the transferability of and (D). The and (D) genes were identified in two strains named SS17 and SS20, respectively. The of the two isolates was located on chromosomes invariably associated with the gene and Tn, which carry the resistance genes (A) and (9). The two plasmids that carry (D), pSS17 (7550 bp) and pSS20-1 (7550 bp) have 100% nucleotide sequence identity. The (D) was flanked by GMP synthase and IS1202. The findings of this study extend the current knowledge of the genetic background of and (D) and indicate that Tn and IS1202 may play an important role in the transmission of and (D), respectively.
本研究旨在调查恶唑烷酮耐药基因和（D）在……中的存在情况及特征。2020 - 2021年期间，从中国养猪场共收集了36株分离株（30株……分离株，6株……分离株），采用聚合酶链反应（PCR）来确定……和……的存在情况。然后，对这36株链球菌分离株中的2株进行如下进一步处理。采用全基因组测序和从头组装来分析……和（D）基因的遗传环境。采用接合试验和反向PCR来验证……和（D）的可转移性。在分别名为SS17和SS20的两株……菌株中鉴定出了……和（D）基因。这两株分离株的……均位于与……基因和Tn始终相关的染色体上，Tn携带耐药基因（A）和（9）。携带（D）的两个质粒，即pSS17（7550 bp）和pSS20 - 1（7550 bp）具有100%的核苷酸序列同一性。（D）两侧是GMP合酶和IS1202。本研究结果扩展了目前关于……和（D）遗传背景的知识，并表明Tn和IS1202可能分别在……和（D）的传播中发挥重要作用。
