da Costa Nayara Souza, Lima Luíza Siqueira, Oliveira Franciele Aparecida Mendes, Galiciolli Maria Eduarda Andrade, Manzano Mariana Inocêncio, Garlet Quelen Iane, Irioda Ana Carolina, Oliveira Cláudia Sirlene
Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80250-060, Brazil.
Faculdades Pequeno Príncipe, Curitiba 80230-020, Brazil.
Biomedicines. 2023 May 3;11(5):1346. doi: 10.3390/biomedicines11051346.
Triple-negative breast cancer (TNBC) is an aggressive, fast-growing tumor that is more likely to spread to distant organs. Among women diagnosed with breast cancer, the prevalence of TNBC is 20%, and treatment is currently limited to chemotherapy. Selenium (Se), an essential micronutrient, has been explored as an antiproliferative agent. Therefore, this study aimed to evaluate the effects of exposure to organic (selenomethionine, ebselen, and diphenyl diselenide) and inorganic (sodium selenate and sodium selenite) Se molecules in different breast cell lines. The compounds were tested at 1, 10, 50, and 100 μM for 48 h in the non-tumor breast cell line (MCF-10A) and TNBC derivatives cell lines (BT-549 and MDA-MB-231). The effects of Se on cell viability, apoptotic and necrotic processes, colony formation, and cell migration were analyzed. Exposure to selenomethionine and selenate did not alter the evaluated parameters. However, selenomethionine had the highest selectivity index (SI). The exposure to the highest doses of selenite, ebselen, and diphenyl diselenide resulted in antiproliferative and antimetastatic effects. Selenite had a high SI to the BT cell line; however, the SI of ebselen and diphenyl diselenide was low in both tumoral cell lines. In conclusion, the Se compounds had different effects on the breast cell lines, and additional tests are needed to reveal the antiproliferative effects of Se compounds.
三阴性乳腺癌（TNBC）是一种侵袭性强、生长迅速的肿瘤，更易扩散至远处器官。在被诊断为乳腺癌的女性中，TNBC的患病率为20%，目前治疗仅限于化疗。硒（Se）作为一种必需的微量营养素，已被研究作为一种抗增殖剂。因此，本研究旨在评估暴露于有机（硒代蛋氨酸、依布硒啉和二苯基二硒化物）和无机（硒酸钠和亚硒酸钠）硒分子对不同乳腺癌细胞系的影响。这些化合物在非肿瘤性乳腺癌细胞系（MCF-10A）和TNBC衍生细胞系（BT-549和MDA-MB-231）中分别以1、10、50和100μM的浓度进行48小时测试。分析了硒对细胞活力、凋亡和坏死过程、集落形成以及细胞迁移的影响。暴露于硒代蛋氨酸和硒酸钠未改变所评估的参数。然而，硒代蛋氨酸具有最高的选择性指数（SI）。暴露于最高剂量的亚硒酸钠、依布硒啉和二苯基二硒化物会产生抗增殖和抗转移作用。亚硒酸钠对BT细胞系具有较高的SI；然而，依布硒啉和二苯基二硒化物在两种肿瘤细胞系中的SI均较低。总之，硒化合物对乳腺癌细胞系有不同影响，需要进一步测试以揭示硒化合物的抗增殖作用。
