Sato Ken, Naganuma Atsushi, Nagashima Tamon, Arai Yosuke, Mikami Yuka, Nakajima Yuka, Kanayama Yuki, Murakami Tatsuma, Uehara Sanae, Uehara Daisuke, Yamazaki Yuichi, Murase Takayo, Nakamura Takashi, Uraoka Toshio
Department of Gastroenterology and Hepatology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Japan.
Department of Hepatology, Heisei Hidaka Clinic, Takasaki 371-0001, Japan.
Biomedicines. 2023 May 14;11(5):1445. doi: 10.3390/biomedicines11051445.
Studies evaluating xanthine oxidoreductase (XOR) activities in comprehensive liver diseases are scarce, and different etiologies have previously been combined in groups for comparison. To accurately evaluate XOR activities in liver diseases, the plasma XOR activities in etiology-based comprehensive liver diseases were measured using a novel, sensitive, and accurate assay that is a combination of liquid chromatography and triple quadrupole mass spectrometry to detect [C, N]uric acid using [C, N]xanthine as a substrate. We also mainly evaluated the association between the plasma XOR activities and parameters of liver tests, purine metabolism-associated markers, oxidative stress markers, and an inflammation marker. In total, 329 patients and 32 controls were enrolled in our study. Plasma XOR activities were generally increased in liver diseases, especially in the active phase, such as in patients with hepatitis C virus RNA positivity, those with abnormal alanine transaminase (ALT) levels in autoimmune liver diseases, and uncured hepatocellular carcinoma patients. Plasma XOR activities were numerically highest in patients with acute hepatitis B. Plasma XOR activities were closely correlated with parameters of liver tests, especially serum ALT levels, regardless of etiology and plasma xanthine levels. Our results indicated that plasma XOR activity might reflect the active phase in various liver diseases.
评估综合肝病中黄嘌呤氧化还原酶（XOR）活性的研究较少，且之前不同病因的研究被合并分组进行比较。为准确评估肝病中的XOR活性，我们采用一种新型、灵敏且准确的检测方法，即液相色谱与三重四极杆质谱联用，以[C,N]黄嘌呤为底物检测[C,N]尿酸，来测定基于病因的综合肝病患者血浆中的XOR活性。我们还主要评估了血浆XOR活性与肝功能检查参数、嘌呤代谢相关标志物、氧化应激标志物及炎症标志物之间的关联。本研究共纳入329例患者和32例对照。肝病患者血浆XOR活性普遍升高，尤其是在活动期，如丙型肝炎病毒RNA阳性患者、自身免疫性肝病中丙氨酸转氨酶（ALT）水平异常的患者以及未治愈的肝细胞癌患者。急性乙型肝炎患者的血浆XOR活性在数值上最高。无论病因和血浆黄嘌呤水平如何，血浆XOR活性均与肝功能检查参数密切相关，尤其是血清ALT水平。我们的结果表明，血浆XOR活性可能反映各种肝病的活动期。
