Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Prolongación de Carpio y Plan de Ayala S/N, Colonia Santo Tomas, Delegación Miguel Hidalgo, Ciudad de México 11340, Mexico.
Departamento de Química Inorgánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional (IPN), Prolongación de Carpio y Plan de Ayala S/N, Colonia Santo Tomas, Delegación Miguel Hidalgo, Ciudad de México 11340, Mexico.
Genes (Basel). 2023 Apr 30;14(5):1023. doi: 10.3390/genes14051023.
The slow-growing, nontuberculous mycobacterium possesses two rRNA operons, and , located downstream from the and genes, respectively. Here, we report the sequence and organization of the promoter regions of these two operons. In the operon, transcription can be initiated from the two promoters, named P1 and PCL1, while in , transcription can only start from one, called P1 . Both operons show a similar organization to the one described in and . Furthermore, by qRT-PCR analyses of the products generated from each promoter, we report that stress conditions such as starvation, hypoxia, and cellular infection affect the contribution of each operon to the synthesis of pre-rRNA. It was found that the products from the PCL1 promoter of play a pivotal role in rRNA synthesis during all stress conditions. Interestingly, the main participation of the products of transcription from the P1 promoter of was found during hypoxic conditions at the NRP1 phase. These results provide novel insights into pre-rRNA synthesis in mycobacteria, as well as the potential ability of to produce latent infections.
生长缓慢、非结核分枝杆菌拥有两个 rRNA 操纵子、和，分别位于和基因的下游。在这里，我们报告了这两个操纵子启动子区域的序列和组织。在操纵子中，转录可以从两个启动子开始，分别命名为 P1 和 PCL1，而在中，转录只能从一个启动子开始，称为 P1。这两个操纵子都显示出与中描述的相似的组织。此外，通过对每个启动子产生的产物进行 qRT-PCR 分析，我们报告说，饥饿、缺氧和细胞感染等应激条件会影响每个操纵子对 pre-rRNA 合成的贡献。结果发现，在所有应激条件下，来自的 PCL1 启动子的产物在 rRNA 合成中起着关键作用。有趣的是，在 NRP1 期缺氧条件下，来自的 P1 启动子转录的产物主要参与。这些结果为分枝杆菌的 pre-rRNA 合成以及的潜在潜伏感染能力提供了新的见解。
