Guangdong Provincial Key Lab of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China.
Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou 510310, China.
Genes (Basel). 2023 May 5;14(5):1042. doi: 10.3390/genes14051042.
The transcription factor IbbHLH2 has been identified as involved in the biosynthesis of anthocyanins in purple-flesh sweet potatoes. However, little is known about the upstream transcription regulators of the promoter of in terms of their involvement in anthocyanin biosynthesis. For this study, the transcription regulators of the promoter of were screened via yeast one-hybrid assays in purple-fleshed sweet potato storage roots. Seven proteins, namely IbERF1, IbERF10, IbEBF2, IbPDC, IbPGP19, IbUR5GT, and IbDRM, were screened as upstream binding proteins of the promoter of . The interactions between the promoter and these upstream binding proteins were verified using dual-luciferase reporter and yeast two-hybrid assays. Furthermore, the gene expression levels of transcription regulators, transcription factors, and structural genes involved in the anthocyanin biosynthesis of different root stages of purple and white-fleshed sweet potatoes were analyzed via real-time PCR. The obtained results indicate that IbERF1 and IbERF10 are key transcription regulators of the promoter of and are involved in anthocyanin biosynthesis in purple-fleshed sweet potatoes.
转录因子 IbbHLH2 已被确定参与了紫色果肉甘薯中花色苷的生物合成。然而，关于其在花色苷生物合成中涉及的启动子上游转录调节剂知之甚少。在这项研究中，通过紫色果肉甘薯贮藏根的酵母单杂交试验筛选了 启动子的转录调节剂。筛选到 7 种蛋白质，即 IbERF1、IbERF10、IbEBF2、IbPDC、IbPGP19、IbUR5GT 和 IbDRM，作为启动子的上游结合蛋白。通过双荧光素酶报告基因和酵母双杂交试验验证了启动子与这些上游结合蛋白之间的相互作用。此外，通过实时 PCR 分析了不同根阶段紫色和白色果肉甘薯花色苷生物合成中涉及的转录调节剂、转录因子和结构基因的基因表达水平。结果表明，IbERF1 和 IbERF10 是 启动子的关键转录调节剂，参与了紫色果肉甘薯中花色苷的生物合成。
