Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China.
Henry Fok School of Biology and Agriculture, Shaoguan University, Shaoguan 512005, China.
Int J Mol Sci. 2023 May 19;24(10):8997. doi: 10.3390/ijms24108997.
Huanglongbing, a globally devastating citrus disease, is associated with Liberibacter asiaticus (Las) and is mainly transmitted by . Verification of the distribution and dynamics of Las in is critical to understanding Las transmitted by vectors in nature. Here, the distribution and titers of Las in different sexes and tissues of adults were investigated by fluorescence in-situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR). Results showed that Las had widespread distribution in the brain, salivary glands, digestive system, and reproductive system of both females and males, indicating a systemic infection of Las in . Moreover, Las fluorescence intensity and titers were significantly increased in both the digestive system and the female reproductive system with development and there was a marked decreased in both the salivary glands and the male brain, but there was no significant change in the female brain or the male reproductive system. Furthermore, the distribution and dynamics of Las in embryos and nymphs were investigated. Las was observed in all laid eggs and subsequent first-second-instar nymphs, indicating that a high percentage of embryos and nymphs resulting from infected mothers were infected with CLas.
黄龙病是一种全球破坏性的柑橘病害，与亚洲韧皮杆菌（Las）有关，主要通过 传播。验证 Las 在 中的分布和动态对于了解自然传播媒介传播的 Las 至关重要。在这里，通过荧光原位杂交（FISH）和定量实时 PCR（qRT-PCR）研究了 Las 在 成虫不同性别和组织中的分布和滴度。结果表明，Las 在雌性和雄性的大脑、唾液腺、消化系统和生殖系统中广泛分布，表明 Las 在 中存在全身性感染。此外，Las 的荧光强度和滴度随着发育在消化系统和雌性生殖系统中显著增加，而在唾液腺和雄性大脑中显著降低，但在雌性大脑或雄性生殖系统中没有明显变化。此外，还研究了 Las 在胚胎和若虫中的分布和动态。Las 存在于所有产下的卵和随后的第一-二龄若虫中，表明感染了 CLas 的 母亲所产生的胚胎和若虫的比例很高。
