Huanglongbing, a globally devastating citrus disease, is associated with Liberibacter asiaticus (Las) and is mainly transmitted by . Verification of the distribution and dynamics of Las in is critical to understanding Las transmitted by vectors in nature. Here, the distribution and titers of Las in different sexes and tissues of adults were investigated by fluorescence in-situ hybridization (FISH) and quantitative real-time PCR (qRT-PCR). Results showed that Las had widespread distribution in the brain, salivary glands, digestive system, and reproductive system of both females and males, indicating a systemic infection of Las in . Moreover, Las fluorescence intensity and titers were significantly increased in both the digestive system and the female reproductive system with development and there was a marked decreased in both the salivary glands and the male brain, but there was no significant change in the female brain or the male reproductive system. Furthermore, the distribution and dynamics of Las in embryos and nymphs were investigated. Las was observed in all laid eggs and subsequent first-second-instar nymphs, indicating that a high percentage of embryos and nymphs resulting from infected mothers were infected with CLas.