MacLusky N J, Roy E J, Shanabrough M, Eisenfeld A J
J Neurosci Methods. 1986 Apr;16(2):131-40. doi: 10.1016/0165-0270(86)90045-2.
This paper examines the effects of freezing and thawing on steroid receptor concentrations in the brain and pituitary of the rat. Storage at -70 degrees C for 1-2 weeks had no detectable effect on levels of cytoplasmic estrogen receptors. However, freezing and thawing resulted in measurable losses of cytoplasmic androgen, progestin and glucocorticoid receptors. Cell nuclear receptors were measured by exchange assay after in vivo administration of non-radioactive steroids. Nuclear estrogen, androgen and progestin receptor concentrations were all reduced by freezing compared to the levels in fresh tissue. In all cases except that of cytoplasmic glucocorticoid receptors, these losses could be prevented by freezing the tissue in 10% aqueous dimethylsulfoxide.
本文研究了冻融对大鼠脑和垂体中类固醇受体浓度的影响。在-70℃储存1-2周对细胞质雌激素受体水平没有可检测到的影响。然而，冻融导致细胞质雄激素、孕激素和糖皮质激素受体出现可测量的损失。通过在体内给予非放射性类固醇后进行交换测定来测量细胞核受体。与新鲜组织中的水平相比，冷冻使细胞核雌激素、雄激素和孕激素受体浓度均降低。除了细胞质糖皮质激素受体外，在所有情况下，将组织冷冻在10%的二甲基亚砜水溶液中可以防止这些损失。
