Huang Cheng, Lin Zi-Jun, Chen Jui-Chieh, Zheng Hao-Jun, Lai Yu-Heng, Huang Hsiu-Chen
Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan.
Center for Teacher Education, National Tsing Hua University, Hsinchu 30014, Taiwan.
Pharmaceuticals (Basel). 2023 May 10;16(5):727. doi: 10.3390/ph16050727.
α-Viniferin, a natural stilbene compound found in plants and a polymer of resveratrol, had demonstrated potential anti-cancer and anti-inflammatory effects. However, the specific mechanisms underlying its anti-cancer activity were not yet fully understood and required further investigation. This study evaluated the effectiveness of α-viniferin and ε-viniferin using MTT assay. Results showed that α-viniferin was more effective than ε-viniferin in reducing the viability of NCI-H460 cells, a type of non-small cell lung cancer. Annexin V/7AAD assay results provided further evidence that the decrease in cell viability observed in response to α-viniferin treatment was due to the induction of apoptosis in NCI-H460 cells. The present findings indicated that treatment with α-viniferin could stimulate apoptosis in cells by cleaving caspase 3 and PARP. Moreover, the treatment reduced the expression of SIRT1, vimentin, and phosphorylated AKT, and also induced AIF nuclear translocation. Furthermore, this research provided additional evidence for the effectiveness of α-viniferin as an anti-tumor agent in nude mice with NCI-H460 cell xenografts. As demonstrated by the TUNEL assay results, α-viniferin promoted apoptosis in NCI-H460 cells in nude mice.
α-葡萄素是一种存在于植物中的天然芪类化合物，也是白藜芦醇的聚合物，已显示出潜在的抗癌和抗炎作用。然而，其抗癌活性的具体机制尚未完全明确，需要进一步研究。本研究采用MTT法评估了α-葡萄素和ε-葡萄素的有效性。结果表明，在降低非小细胞肺癌NCI-H460细胞活力方面，α-葡萄素比ε-葡萄素更有效。膜联蛋白V/7-氨基放线菌素D检测结果进一步证明，α-葡萄素处理后观察到的细胞活力下降是由于NCI-H460细胞凋亡所致。目前的研究结果表明，α-葡萄素处理可通过切割半胱天冬酶3和聚（ADP-核糖）聚合酶来刺激细胞凋亡。此外，该处理降低了沉默信息调节因子1、波形蛋白和磷酸化蛋白激酶B的表达，还诱导了凋亡诱导因子的核转位。此外，本研究为α-葡萄素作为NCI-H460细胞异种移植裸鼠的抗肿瘤药物的有效性提供了更多证据。TUNEL检测结果表明，α-葡萄素可促进裸鼠体内NCI-H460细胞的凋亡。
