Laboratory of Microbial Biotechnology, Eliava Institute of Bacteriophages, Microbiology and Virology, Tbilisi 0160, Georgia.
Department of Biology, Faculty of Exact and Natural Sciences, Javakhishvili Tbilisi State University, Tbilisi 0179, Georgia.
Viruses. 2023 Apr 25;15(5):1042. doi: 10.3390/v15051042.
is a commensal gut bacterium that is associated with a number of blood and tissue infections. It has not yet been recognized as one of the drug-resistant human pathogens, but cases of the refractory infections, caused by strains that are not susceptible to the common antibiotic regimes established for have been more frequently reported. Bacteriophages (phages) were found to be a successful antibacterial alternative to antibiotic therapy in many cases of multidrug-resistant (MDR) bacterial infections. We have characterized the bacteriophage GEC_vB_Bfr_UZM3 (UZM3), which was used for the treatment of a patient with a chronic osteomyelitis caused by a mixed infection. Studied biological and morphological properties of UZM3 showed that it seems to represent a strictly lytic phage belonging to a siphovirus morphotype. It is characterized by high stability at body temperature and in pH environments for about 6 h. Whole genome sequencing analysis of the phage UZM3 showed that it does not harbor any known virulence genes and can be considered as a potential therapeutic phage to be used against infections.
是一种共生肠道细菌，与许多血液和组织感染有关。它尚未被认为是耐药性人类病原体之一，但越来越多的报道称，由对抗生素治疗方案不敏感的菌株引起的难治性感染病例。噬菌体（phages）在许多多重耐药（MDR）细菌感染的情况下被发现是一种替代抗生素治疗的成功抗菌方法。我们已经对噬菌体 GEC_vB_Bfr_UZM3（UZM3）进行了表征，该噬菌体用于治疗一名慢性骨髓炎患者，该患者的感染由混合感染引起。对 UZM3 的生物学和形态学特性进行的研究表明，它似乎代表一种严格的裂解噬菌体，属于丝状噬菌体形态型。它在体温和 pH 环境下约 6 小时内具有高度稳定性。噬菌体 UZM3 的全基因组测序分析表明，它不携带任何已知的毒力基因，可以被认为是一种潜在的治疗性噬菌体，可用于对抗感染。
