Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville BP 769, Gabon.
Institut National Supérieur d'Agronomie et de Biotechnologies (INSAB), Université des Sciences et Techniques de Masuku (USTM), Franceville BP 913, Gabon.
Viruses. 2023 May 15;15(5):1169. doi: 10.3390/v15051169.
The first detection of canine parvovirus type-2 (CPV-2) was in the early 1970s, when it was known to cause severe gastroenteritis in dogs. However, it has evolved over the years into CPV-2a within 2 years, into CPV-2b after 14 years, into CPV-2c after 16 years and more recently CPV-2a-, 2b- and 2c-like variants reported in 2019, with a global distribution. Reports on the molecular epidemiology of this virus are missing in most African countries. The report of clinical cases among vaccinated dogs in Libreville in Gabon triggered the execution of this study. The objective of this study was to characterize circulating variants from dogs showing clinical signs suggestive of CPV that were examined by a veterinarian. A total of eight (8) fecal swab samples were collected, and all had positive PCR results. Sequencing, Blast analysis and assembly of two whole genomes and eight partial VP2 sequences were performed, and the sequences submitted to GenBank. Genetic characterization revealed the presence of CPV-2a and CPV-2c variants with predominance of the former. Phylogenetically, the Gabonese CPVs formed distinct groups similar to Zambian CPV-2c and Australian CPV-2a sequences. The antigenic variants CPV-2a and CPV-2c have not yet been reported in Central Africa. However, these CPV-2 variants circulate in young, vaccinated dogs in Gabon. These results suggest additional epidemiological and genomic studies are required in order to evaluate the occurrence of different CPV variants in Gabon and effectiveness of the commercial vaccines used against protoparvovirus in the country.
犬细小病毒 2 型（CPV-2）的首次检测是在 20 世纪 70 年代早期，当时它被认为会导致犬严重的胃肠炎。然而，它在过去的几十年中不断进化，2 年内演变成 CPV-2a，14 年后演变成 CPV-2b，16 年后演变成 CPV-2c，最近在 2019 年又报道了 CPV-2a、2b 和 2c 样变体，在全球范围内分布。大多数非洲国家都没有关于该病毒分子流行病学的报告。加蓬利伯维尔接种疫苗的犬出现临床病例的报告引发了这项研究的执行。本研究的目的是对兽医检查显示出犬细小病毒临床症状的循环变异体进行特征描述。共采集了 8 个粪便拭子样本，所有样本的 PCR 结果均为阳性。对两个全基因组和 8 个部分 VP2 序列进行了测序、Blast 分析和组装，并将序列提交给 GenBank。遗传特征表明，存在 CPV-2a 和 CPV-2c 变异体，前者占优势。系统进化分析表明，加蓬 CPV 与赞比亚 CPV-2c 和澳大利亚 CPV-2a 序列形成了不同的群体。抗原变异体 CPV-2a 和 CPV-2c 尚未在中非报告过。然而，这些 CPV-2 变异体在加蓬的年轻、接种疫苗的犬中传播。这些结果表明，需要进一步开展流行病学和基因组研究，以评估不同 CPV 变异体在加蓬的发生情况以及该国使用的针对细小病毒的商业疫苗的有效性。
