用氚标记的螺哌隆或N-正丙基去甲阿朴吗啡的示踪剂和最大特异性结合来定量大鼠脑区体内的多巴胺受体。

Tracer and maximal specific binding of tritiated spiperone or N-n-propylnorapomorphine to quantify dopamine receptors in rat brain regions in vivo.

作者信息

Van der Werf J F, Sebens J B, Korf J

出版信息

Life Sci. 1986 Jul 14;39(2):155-60. doi: 10.1016/0024-3205(86)90450-9.

Abstract

Specific tracer and maximal specific binding (Bmax) were determined in rat brain regions from radioactivity accumulation after intravenous administration of 3H N-n-propylnorapomorphine (NPA) or 3H spiperone at various specific activities. With NPA the highest Bmax-values (expressed in pmol.g-1 tissue) were found in the striatum (26 pmol.g-1) nucleus accumbens (about 27 pmol.g-1) and the olfactory tubercle (11 pmol.g-1). Saturable NPA binding was also found in the amygdaloid complex, medulla oblongata and inferior colliculi, but not in the frontal cortex. Bmax values for spiperone were high in the striatum (73 pmol.g-1), the nucleus accumbens (48 pmol.g-1), the olfactory tubercle (34 pmol.g-1) and the frontal cortex (18 pmol.g-1). A similar order was found for the tracer contents in these regions. There was no linear relationship between these contents and Bmax values. The possible implications of these findings and usefulness of NPA for brain imaging are discussed.

摘要

在大鼠脑区中，通过静脉注射不同比活度的³H N-正丙基去甲阿扑吗啡（NPA）或³H 螺哌隆后放射性积累来测定特异性示踪剂和最大特异性结合（Bmax）。使用 NPA 时，在纹状体（26 pmol·g⁻¹ 组织）、伏隔核（约 27 pmol·g⁻¹）和嗅结节（11 pmol·g⁻¹）中发现了最高的 Bmax 值（以 pmol·g⁻¹ 组织表示）。在杏仁复合体、延髓和下丘脑中也发现了可饱和的 NPA 结合，但在额叶皮质中未发现。螺哌隆的 Bmax 值在纹状体（73 pmol·g⁻¹）、伏隔核（48 pmol·g⁻¹）、嗅结节（34 pmol·g⁻¹）和额叶皮质（18 pmol·g⁻¹）中较高。在这些区域中示踪剂含量也发现了类似的顺序。这些含量与 Bmax 值之间没有线性关系。讨论了这些发现的可能意义以及 NPA 在脑成像中的有用性。