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N-糖组学分析为鱼肉质地形成的分子机制提供了新的见解。

N-glycosylomic analysis provides new insight into the molecular mechanism of firmness of fish fillet.

机构信息

Key Laboratory of Aquatic Animal Immune Technology of Guangdong Province, Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China.

BGI Genomics, BGI-Shenzhen, Shenzhen, China.

出版信息

Food Chem. 2023 Oct 30;424:136417. doi: 10.1016/j.foodchem.2023.136417. Epub 2023 May 20.

Abstract

Post-translational protein modification affects muscle physiochemistry. To understand the roles of N-glycosylation in this process, the muscle N-glycoproteomes of crisp grass carp (CGC) and ordinary grass carp (GC) were analyzed and compared. We identified 325 N-glycosylated sites with the NxT motif, classified 177 proteins, and identified 10 upregulated and 19 downregulated differentially glycosylated proteins (DGPs). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes annotations revealed that these DGPs participate in myogenesis, extracellular matrix content formation, and muscle function. The DGPs partially accounted for the molecular mechanisms associated with the relatively smaller fiber diameter and higher collagen content observed in CGC. Though the DGPs diverged from the identified differentially phosphorylated proteins and differentially expressed proteins detected in previous study, they all shared similar metabolic and signaling pathways. Thus, they might independently alter fish muscle texture. Overall, the present study provides novel insights into the mechanisms underlying fillet quality.

摘要

翻译后文本

蛋白质翻译后修饰影响肌肉的生理化学特性。为了理解 N-糖基化在此过程中的作用,我们对脆肉鲩和普通草鱼的肌肉 N-糖蛋白组进行了分析和比较。我们鉴定出了具有 NxT 基序的 325 个 N-糖基化位点,对 177 种蛋白质进行了分类,并鉴定出了 10 个上调和 19 个下调的差异糖基化蛋白 (DGPs)。GO 和 Kyoto Encyclopedia of Genes and Genomes 注释表明,这些 DGP 参与了肌发生、细胞外基质成分的形成和肌肉功能。DGP 部分解释了在脆肉鲩中观察到的纤维直径较小和胶原蛋白含量较高的分子机制。尽管 DGP 与之前研究中检测到的差异磷酸化蛋白和差异表达蛋白不同,但它们都共享相似的代谢和信号通路。因此,它们可能独立地改变鱼类肌肉的质地。总的来说,本研究为鱼片质量的机制提供了新的见解。

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