Department of Zoology, School of Biosciences, Avinashilingam Institute for Home Science and Higher Education for Women, Coimbatore, Tamil Nadu, 641043, India.
Mol Biotechnol. 2024 Mar;66(3):500-516. doi: 10.1007/s12033-023-00764-5. Epub 2023 May 28.
The efficiency of Cladophora species for the removal of Reactive Orange 107 (RO107) from the aqueous solution was evaluated through batch adsorption studies by optimising various process parameters such as pH (3-8), dye concentration (100-500 mg/l), biosorbent concentration (100-500 mg/l), temperature (25-45 °C) and contact time (12-108 h). The results revealed that the optimum conditions for RO107 decolourisation (87%) was found on 72 h of incubation with 100 mg/l dye concentration amended with 200 mg/l biosorbent at pH 6 at 25 °C. The mechanism of dye adsorption was evaluated using isotherms, kinetics and thermodynamic models. The experimental data fitted well with Langmuir isotherm and pseudo-second-order kinetic models. Thermodynamic studies revealed that the adsorption process was endothermic, spontaneous and feasible in nature. Recovery of RO107 from the Cladophora sp. was maximum when 0.1 M HNO was used as an eluent. UV-Visible, FT-IR and SEM analyses reveal the interaction between the biosorbent-adsorbate and confirm the process of decolourisation by Cladophora sp. In order to evaluate the nature of the untreated and treated dye solutions, toxicological studies were conducted and the results revealed that the treated dye solution was non- toxic as compared with untreated dye solution. The results of the docking study proved that there was a substantial binding energy between RO107 and the protein (Cytochrome C6) of Cladophora sp. Hence, Cladophora sp. proves to be a promising biosorbent to decolourise RO107 and its potential can be explored in the textile sectors.
采用批量吸附实验，评估了石莼属（Cladophora）物种对活性艳橙 107（RO107）从水溶液中去除的效率，通过优化各种工艺参数，如 pH（3-8）、染料浓度（100-500mg/L）、生物吸附剂浓度（100-500mg/L）、温度（25-45°C）和接触时间（12-108h）。结果表明，在 pH 值为 6、25°C 的条件下，用 100mg/L 染料浓度和 200mg/L 生物吸附剂孵育 72h 后，RO107 的脱色率（87%）达到最佳条件。采用等温线、动力学和热力学模型评估了染料吸附的机理。实验数据与朗缪尔等温线和拟二级动力学模型拟合良好。热力学研究表明，吸附过程是吸热的、自发的和可行的。当使用 0.1M HNO 作为洗脱剂时，从石莼属中回收 RO107 的效率最高。UV-可见、FT-IR 和 SEM 分析表明了生物吸附剂-吸附物之间的相互作用，并证实了石莼属对染料的脱色过程。为了评估未经处理和处理后的染料溶液的性质，进行了毒理学研究，结果表明，与未经处理的染料溶液相比，处理后的染料溶液是非毒性的。对接研究的结果证明，RO107 与石莼属（Cladophora sp.）的蛋白质（细胞色素 C6）之间存在显著的结合能。因此，石莼属（Cladophora sp.）被证明是一种有前途的生物吸附剂，可用于对 RO107 进行脱色，其潜力可在纺织行业中得到探索。
