Walsh M T, Atkinson D
Methods Enzymol. 1986;128:582-608. doi: 10.1016/0076-6879(86)28093-3.
The methodologies described here for the selective and sequential reassembly of model LDL particles, although in many instances still in the developmental stages, will undoubtedly provide a basis on which further advances in LDL reassembly will be made. Reassembled LDL complexes of defined lipids and apoB provide well-defined model systems in which to study the molecular interactions and structural organization of LDL, including the lipid-lipid interactions in the particle core, the lipid-lipid and lipid-protein interactions which determine the surface organization and protein conformation, and the interactions between the core and surface components. These reassembled LDL complexes should serve as important models to study the delivery of isotopically labeled lipids with differing physical properties to cells in order to investigate the metabolic complexity of intracellular LDL catabolism and its relationship to positive cholesterol balance and atherogenesis.
本文所述的用于模型低密度脂蛋白（LDL）颗粒选择性和顺序性重新组装的方法，尽管在许多情况下仍处于发展阶段，但无疑将为LDL重新组装的进一步进展提供基础。具有明确脂质和载脂蛋白B的重新组装LDL复合物提供了定义明确的模型系统，用于研究LDL的分子相互作用和结构组织，包括颗粒核心中的脂质-脂质相互作用、决定表面组织和蛋白质构象的脂质-脂质和脂质-蛋白质相互作用，以及核心与表面成分之间的相互作用。这些重新组装的LDL复合物应作为重要模型，用于研究将具有不同物理性质的同位素标记脂质递送至细胞，以研究细胞内LDL分解代谢的代谢复杂性及其与正胆固醇平衡和动脉粥样硬化的关系。
