过氧化氢酶基因 C-262 多态性与类风湿关节炎的关联。

Association of C-262 polymorphism in catalase gene with rheumatoid arthritis.

机构信息

National University of Medical Sciences, Rawalpindi.

Department of Biochemistry & Molecular Biology, Army Medical College, National University of Sciences & Technology, Rawalpindi, Pakistan.

出版信息

J Pak Med Assoc. 2022 Dec;72(12):2404-2408. doi: 10.47391/JPMA.3451.

DOI:10.47391/JPMA.3451

PMID:37246657

Abstract

OBJECTIVE

To evaluate the association of C-262 polymorphism in Catalase gene (CAT) with Rheumatoid Arthritis.

METHODS

The comparative cross-sectional study was conducted at the Department of Biochemistry and Molecular Biology, Army Medical College, Rawalpindi, in collaboration with the Rheumatology Department, Pak Emirates Military Hospital, Rawalpindi, Pakistan, from January to December 2020, and comprised Deoxyribonucleic acid extraction of samples. Samples in group I belonged to diagnosed rheumatoid arthritis patients of either gender aged 30-60 years who were on disease-modifying anti-rheumatic drugs. Group II had an equal number of healthy controls. The promoter region of the CAT gene having the polymorphic segment was amplified through polymerase chain reaction, and its products were then subjected to restriction fragment length polymorphism for the analysis of polymorphic region of the CAT gene. Genotypic frequency equilibrium and the association of polymorphism with rheumatoid arthritis was checked. Also, association between fasting lipid profile and haemoglobin was assessed. Data was analysed using SPSSS 22.

RESULTS

Of the 60 samples, 30(50%) belonged to each of the two groups. The mean age was 44.90±10.50 years (range: 30-60 years). Overall, there were 34(56.7%) males and 26(43.3%) were females. Two alleles and three genotypes of the polymorphism was detected. The frequency of CC genotype was higher in group I 23(76.6%), but no association of any of the genotype of polymorphism was found significant (p <0.05). Haemoglobin and lipid profile levels were significantly different in the two groups (p≤0.05).

CONCLUSIONS

There was no significant association found between C-262 polymorphism in CAT gene and rheumatoid arthritis.

摘要

目的

评估过氧化氢酶基因（CAT）C-262 多态性与类风湿关节炎的关联。

方法

本研究为 2020 年 1 月至 12 月在巴基斯坦拉瓦尔品第的陆军军医大学生化与分子生物学系与拉瓦尔品第的 Pak Emirates Military Hospital 的风湿病科合作进行的病例对照横断面研究，包括样本脱氧核糖核酸提取。第 I 组样本为年龄在 30-60 岁之间的男女确诊类风湿关节炎患者，他们正在服用改善病情的抗风湿药物。第 II 组有相同数量的健康对照者。通过聚合酶链反应扩增 CAT 基因启动子区域的多态性片段，然后对其产物进行限制性片段长度多态性分析以研究 CAT 基因的多态性区域。检查基因频率平衡和多态性与类风湿关节炎的关联。还评估了空腹血脂谱与血红蛋白之间的关系。使用 SPSSS 22 对数据进行分析。

结果

在 60 个样本中，两组各有 30 个（50%）。平均年龄为 44.90±10.50 岁（范围：30-60 岁）。总体而言，有 34 名（56.7%）男性和 26 名（43.3%）女性。该多态性检测到两种等位基因和三种基因型。第 I 组 CC 基因型的频率较高（23 例，76.6%），但多态性的任何基因型均与类风湿关节炎无关（p<0.05）。两组血红蛋白和血脂谱水平差异显著（p≤0.05）。