烧伤后伤口愈合过程中单核细胞和细胞因子的动态变化。

The dynamic changes of monocytes and cytokines during wound healing post-burn injury.

机构信息

Wound Healing Research Group, Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada.

Wound Healing Research Group, Division of Plastic and Reconstructive Surgery, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada; Division of Critical Care, University of Alberta, Edmonton, Alberta, Canada.

出版信息

Cytokine. 2023 Aug;168:156231. doi: 10.1016/j.cyto.2023.156231. Epub 2023 May 27.

DOI:10.1016/j.cyto.2023.156231

PMID:37247448

Abstract

BACKGROUND

Burn injury is a sudden and traumatic injury that affects a large part of the population worldwide, who are placed at high risk of developing hypertrophic scars (HTS). HTS are a fibrotic scar resulting in painful contracted and raised scarring, affecting mobility in joints and work life, as well as cosmetically. The aim of this research was to enhance our understanding of the systematic response of monocytes and cytokines in wound healing after burn injury, in order to develop novel approaches to prevention and treatment of HTS.

METHODS

Twenty-seven burn patients and thirteen healthy individuals were recruited in this study. Burn patients were stratified by burn total body surface area (TBSA). Peripheral blood samples were taken post-burn injury. Serum and peripheral blood mononuclear cells (PBMCs) were separated from the blood samples. This research investigated cytokines IL-6, IL-8, IL1RA, IL-10, and chemokine pathways SDF-1/CXCR4, MCP-1/CCR2, RANTES/CCR5 during the wound healing process in burn patients with varying severity of injuries by using enzyme-linked immunosorbent assays. PBMCs were stained for monocytes and the chemokine receptors by flow cytometry. Statistical analysis was done by one-way ANOVA with a Tukey correction, and regression analysis was performed using Pearson's Correlation analysis.

RESULTS

The CD14 CD16 monocyte subpopulation is larger in patients who developed HTS at 4-7 days. The CD14 CD16 monocyte subpopulation is smaller in the first week of injury, where it is similar after 8 days. Burn injury increased CXCR4, CCR2, and CCR5 expressions in CD14 CD16 monocytes. Increases in MCP-1 at 0-3 days after burn injury was positively correlated with burn severity. IL-6, IL-8, RANTES, and MCP-1 significantly increased with increasing burn severity.

CONCLUSIONS

Monocytes and their chemokine receptors, as well as systemic levels of cytokines in wound healing of burn patients and scar development will require ongoing assessment to enhance our understanding of the abnormal wound healing after burn injury.

摘要

背景

烧伤是一种突发的创伤，影响着全球很大一部分人群，使他们面临发生增生性瘢痕（HTS）的高风险。HTS 是一种纤维性瘢痕，导致疼痛性挛缩和隆起的瘢痕，影响关节的活动能力和工作生活，以及外观。本研究旨在提高我们对烧伤后伤口愈合过程中单核细胞和细胞因子系统反应的理解，以便开发预防和治疗 HTS 的新方法。

方法

本研究纳入了 27 名烧伤患者和 13 名健康个体。根据烧伤总面积（TBSA）对烧伤患者进行分层。在烧伤后采集外周血样本。从血液样本中分离血清和外周血单核细胞（PBMC）。本研究通过酶联免疫吸附试验，研究了不同严重程度烧伤患者在伤口愈合过程中细胞因子 IL-6、IL-8、IL1RA、IL-10 以及趋化因子途径 SDF-1/CXCR4、MCP-1/CCR2、RANTES/CCR5 的变化。通过流式细胞术对 PBMC 进行单核细胞和趋化因子受体染色。采用单因素方差分析（ANOVA）和 Tukey 校正进行统计分析，并采用 Pearson 相关分析进行回归分析。

结果

在烧伤后 4-7 天发生 HTS 的患者中，CD14 CD16 单核细胞亚群更大。在损伤后的第一周，CD14 CD16 单核细胞亚群较小，8 天后则相似。烧伤增加了 CD14 CD16 单核细胞中 CXCR4、CCR2 和 CCR5 的表达。烧伤后 0-3 天 MCP-1 的增加与烧伤严重程度呈正相关。IL-6、IL-8、RANTES 和 MCP-1 随着烧伤严重程度的增加而显著增加。